Karteikarten
Micro Mini Practical 2
Karten in diesem Stapel (47)
- 1—Staphylococcus aureus: Gram positive
(after applying crystal violet) - 2—Escherichia coli: Gram negative
(after applying crystal violet)
- 3—Staphylococcus aureus: Gram positive
(after Gram's iodine)
- 4—Escherichia coli: Gram negative
(after Gram's iodine)
- 5—Staphylococcus aureus: Gram positive
(after acetone alcohol decolorizer)
- 6—Escherichia coli: Gram negative
(after acetone alcohol decolorizer)
- 7—Staphylococcus aureus: Gram positive
(after Safranin counterstain)
- 8—Escherichia coli: Gram negative
(after Safranin counterstain)
- 9In order, what are the 4 steps in the staining procedure?Smearing
Air drying
Heat fixing
Staining - 10Broth samples are easier to prepare, but...harder to see
- 11Agar samples are harder to prepare, but...easier to see
- 12To smear on an Agar sample, you have to...put a drop of water on the slide
- 13Heat fixing is used toaffix the bacteria to the slide
- 14To heat fix a slide...pass it over the flame, don't hold it there.
Overheating will cause it not
to stain correctly - 15Gram Stain Procedure: 4 stepsCrystal Violet
Gram's Iodine
Acetone Alcohol decolorizer
Safranin counterstain
Rinse w/water after each step - 16Ziehl-Neelson Acid-Fast Stains looks for the cell walls of what two types of bacteria?Mycobacterium
and
Nocardia - 17Ziehl-Neelson Acid-Fast Stains steps:Steaming tray
Carbolfuchsin ZN stain (5 min)
Acid-alcohol decolorizer
Loefflers methylene blue counterstain - 18Mycobacterium and Nocardia in an acid-fast stain will turn what color and why?Pink due to the carbolfuchsin dye
- 19antibiotic resistant bacteria and chemical resistance bacteria are gram ________?Gram negative
- 20environmental resistant bacteria heat pH osmotic pressure are gram __________?Gram positive
- 21The difference in gram stains is due to...the difference in the thickness of the cell walls of the bacteria being stained
- 22Specific structures like flagella and capsules are seen usingspecial stains
- 23Gram stains, acid-fast stains, & endospore stains are all...differential stains
- 24Endospore stains use ______ as the primary stain?Malachite green
- 25What stain uses water as the decolorizer?Endospore
- 26Endospore stain steps:Smear, air dry, heat fix
Stain w/ malachite green
(water as decolorizer)
Safranin as decolorizer & counterstain - 27—Bacillus subtilis
(endospore stain)
- 28—Endospore stain
Left - water rinse, followed by Safranin
Right - water rinse, no Safranin
- 29Capsule stains are viewed under what magnification?100x w/oil
- 30What stain does not use heat fix?Capsule stain bc it would
destroy the capsule - 31Malachite Green Safranin Crystal Violet & Methylene Blue are what types of dyes?Cationic dyes (positive sticking to negative particles)
Bacteria have a negative charge, making cationic dyes best - 32Eosin and acid fuchsin are what types of dyes?anionic dyes (negative sticking to positive particles)
- 33Refers to placing a thin layer of the organism on a slideSmear
- 34Allows the organism to dry through evaporationair dry
- 35allows the organism to be attached to the slidefixing
- 36Who developed the Gram StainHans Christian Gram (late 1800s)
- 37What is the very first thing done before Gram Staining?Streaking for isolation
- 38What acts as a mordant (dye fixative)?Gram's Iodine
- 39How can time affect a gram stain?If decolorizing for too long, all bacteria would decolorize and would make it a false gram negative.
If too short, not sufficient, so false gram positive - 40—Capsule stain
- 41How can age affect a gram stain?Cultures older than 24 hours decolorize easier and are falsely Gram negative
- 42How can heat affect a gram stain?The cell wall can be destroyed which leads to a false gram negative
- 43What two bacteria normally construct an endospore?Bacillus & Clostridium
- 44Tetanus, botulism, gangrene, & anthrax ae caused by what bacterias?Bacillus & Clostridium
- 45The pink bacteria seen after an acid-fast stain are read as gram negative? True or FalseFalse
- 46Old cultures tend to stain Gram negative? True or FalseTrue
- 47In the capsule stain, the capsule appears blue in color due to the use of Loafers methylene blue as the primary dye. True of False?False
Informationen
- Beschreibung
- Microbiology Karteikarten am Micro Mini Practical 2, erstellt von Ami G. am 24/02/2022.
- Erstellt von
- Ami G.
- Aufrufe
- 3
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