Karteikarten
Practicals
Karten in diesem Stapel (31)
- 1What is the affinity of methylene blue?Basophilic
- 2What structures are stained by methylene blue and what colour are they stained?Nuclear proteins are stained blue
- 3What is the affinity of haematoxylin?Basophilic
- 4What structure are stained by haematoxylin and what colour are they stained?Nucleic acids: chromatin in the cell nucleus and RNA in the cytoplasm are stained bright orange pink
- 5What is the affinity of eosin Y?Acidophilic
- 6What structure are stained by eosin y and what colour are they stained?Intracellular and extracellular proteins especially in the cytoplasm are stained bright orange pink
- 7What is the affinity of Leishman's stain?Mixed
- 8What structures are stained by leishman's stain and what colour are they stained?Used to identify blood cells. Purple nuclei and granules and pink cytoplasm
- 9In a microscope what is the eyepiece and what does is do?It is a cylinder containing two or more lenses. Its function is to bring the image into focus for the eye.
- 10What does the revolving nosepiece do?Holds multiple objective lenses
- 11What do the objective lenses do?Collect light from the samples
- 12In a microscope what is the role of the condenser?It is a lense designed to focus light from the illumination source onto the sample
- 13In a microscope what does the diaphragm do?Helps to manage the quality and intensity of the illumination
- 14What is the limitation of light microscopes?They can't resolve details of objects that are less than 200nm
- 15Why is the resolution of light microscopes limited?Due to the wavelength of visible light
- 16Why do electron microscopes have a greater resolution than light microscopes?Electrons have a much shorter wavelength than visible light
- 17What is the difference between TEM and SEM microscopes?TEM microscopes produce a 2D image with a very high resolution. SEM produce 3D images with a poorer resolution.
- 18How do you prepare a specimen to be viewed under an electron microscope?The specimen must be completely dried out (dead) because water boils at room temperature in a vacuum
- 19Why do electron microscopes contain a vacuum?Air particles would interfere with the beam of electrons.
- 20In electron microscopes why must the specimen be dyed with an electron dense chemical?Specimens are usually transparent to electrons
- 21What is histopathology?The microscopic examination of cells and tissues for signs of disease
- 22How is dye drawn across a slide?Capillary action
- 23—Basophil- Allergic reaction
- 24—Eosinophil- kills parasites
- 25—Neutrophil- Phagocyte
- 26—Small lymphocyte- Phagocyte
- 27—Monocyte
- 28What are immunostains?Antibodies designed to label specific proteins or cell structures . The antibodies have fluorescent tags which glow under a fluorescent light source.
- 29What is the structure of cheek cells?Flat for surface covering and they tightly fit together (aggregate)
- 30What is the structure of red blood cells?They are designed for transport. Absences of a nucleus allows maximal cytoplasm space and increases flexibility to pass through blood vessels
- 31What is the structure of white blood cells?Large for phagocytosis
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- Biology- Semester 1 (Practicals) Karteikarten am Practicals , erstellt von emma_moran am 08/01/2014.
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