DNA Replication

DNA Replication

1. DNA Replication commences at the Origin and is semi-conservative.

   • 1. DNA Helicase unwinds the DNA strand at the replication fork.
     • 2. DNA Primase (RNA primer) creates a primer on the leading and lagging strands.
       • 3. DNA Polymerase III adds free nucleotides to the leading and lagging strand
         • 4. On the lagging strand Okazaki fragments are produced. DNA Polymerase I exerts exonuclease activity on the Okazaki fragments and removes the primer
           • 5. DNA Ligase seals the nick between the fragments on the lagging strand.
   • OriC in prokaryotes