Quiz
Microbiology Test 1 2nd Year PMU
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Questions in this quiz (90)
1Name the components of nutrient media
- AGlucose Broth
- BMeat-Peptone Broth
- CMeat-Peptone agar
- DBlood Agar
2Most of the bacterial colonies are visualized after:
- A20 minutes
- B2 hours
- C1-2 days
- D1 week
3According to their cell wall structures bacteria divide into
- AAerobes and Anarobes
- BProkaryotes and Eukaryotes
- CGram (+) and Gram (-)
- DPrototrophs and Auxotrophs
4Extra chromosomal genetic elements in bacteria are
- AMesosomes
- BPhagosomes
- CTransposons
- DPlasmids
5How many phases have growth curve of bacteria?
Ans: [blank_start]4[blank_end]
Name them:
a/ [blank_start]Lag[blank_end] Phase
b/ [blank_start]Log[blank_end] Phase
c/ [blank_start]Stationary[blank_end] Phase
d/ [blank_start]Decline[blank_end] Phase- A4
- BLag
- CLog
- DStationary
- EDecline
6Sex-pili of bacteria helps for their
- AMovement
- BConjugation
- CNutrition
- DProtection
7Name the three shapes of bacteria:
a/ [blank_start]Round[blank_end] ([blank_start]Cocci[blank_end])
b/ [blank_start]Rods[blank_end]
c/ [blank_start]Spiral[blank_end] ([blank_start]Spirilli[blank_end])- ARound
- BCocci
- CRods
- DSpiral
- ESpirilli
8What is the most resistant to an unappropiate conditions bacterial form?
- AVegetative
- BSpore
9Which of the following bacterial structures prevents phagocytosis?
- ACytoplasm
- BPeptidoglycan
- CCapsule
- DNuclues
10Are flagellas an obligatory structure of bacterium?
- ATrue
- BFalse
11What chemical is responsible for the acid-fast property of mycobacteria?
Ans: [blank_start]Mycolic acids[blank_end]- AMycolic acids
12Name two bacteria that have capsules:
a/ [blank_start]E. coli[blank_end]
b/ [blank_start]Salmonella[blank_end]- AE. coli
- BSalmonella
13In microbiology what is the most commonly used objective
Ans: [blank_start]Oil[blank_end]
Explain:
This is because [blank_start]oil[blank_end] unlike other liquids like water has very [blank_start]little light refraction[blank_end]- AOil
- Boil
- Clittle light refraction
14What is the name of staining method for Corynebacterium diphtheriae?
- AGram
- BMoller
- CZhiehl-Neelsen
- DNeisser
15Gram (+) bacteria are stained in [blank_start]blue[blank_end] colour and Gram (-) in [blank_start]red[blank_end] colour
- Ablue
- Bred
16The main taxonomic category for bacteria is
- AGenus
- BSpecies
- CFamily
- DOrdo
17According Gram, the Gram (-) bacteria are stained in [blank_start]red[blank_end] colour
- Ared
18The unit for bacterial size is:
- Amilimeter
- Bmicrometer
- Cnanometer
- Dangstrom
19Vibrions are curved bacteria with
- A4-6 curves
- Bmany curves
- Cone curve
- Dtwo curves
20Ziehl-Neelsen is a staining procedure in:
- Aencapsulated bacteria
- Bspore-forming bacteria
- Cacid-fast bacteria
- Dmotile bacteria
21Bacterial cytoplasm contains:
- Acapsule
- Bribosomes
- Cnucleotide
- Dpeptidoglycan
- Epili
- Finclusions
22Name the purposes of simple staining:
a/ Determine cell [blank_start]shape[blank_end]
b/ Determine cell [blank_start]size[blank_end]
c/ Determine cell [blank_start]arrangement[blank_end]- Ashape
- Bsize
- Carrangement
23The main staining procedure that detect morphology of bacteria is:
- ALoeffler
- BNeisser
- CGram
- DPfeiffer
24The name Baccilus antracis defines certain bacterial
- AGenus
- BSpecies
- CClass
25Is it true: Bacterial spores are formed in unfavorable conditions and are means of multiplying
- ATrue
- BFalse
26Staining according Victoria blau is a [blank_start]simple[blank_end] method
- Asimple
- Bcomplex
27Which of the following organelles are essential for bacteria?
- Acytoplasm membrane
- Bpili
- Ccapsule
- Dflagella
- Enucleoid
- Fribosomes
28What are the curved bacteria?
- Astaphylococci
- Bhelicobacter
- Cvibrions
- Dbacilli
29Diplococci are [blank_start]round[blank_end] shaped bacteria that are arranged in [blank_start]pairs[blank_end]
- Around
- Bpairs
30Staphylococci are round shaped bacteria arranged in [blank_start]clusters[blank_end]
- Aclusters
31The volutine bodies are inclusions in the cytoplasm of:
- Amenigococci
- Bdiptheria bacteria
- Cgenococci
- Dpneumococci
32Where is lipopolysaccaride situated?
- Ain the cell wall of Gram (+) bacteria
- Bin the cell wall of Gram (-) bacteria
- Cin the both groups
33Name at least two rod-shaped bacteria
a/ [blank_start]Bacillus anthracis[blank_end]
b/ [blank_start]Clostridium tetani[blank_end]- ABacillus anthracis
- BClostridium tetani
34How many spores form one bacteria?
- Aone
- Btwo
- Cthree
- Dmore than one
35Loefler technique stain bacteria in [blank_start]blue[blank_end] colour
- Ablue
36The causative agent of tuberculosis is stained in red color, using [blank_start]Ziehl[blank_end] [blank_start]Neelsen[blank_end] (name the staining method)
- AZiehl
- BNeelsen
37What are the two purposes of heat fixation
a/ [blank_start]kill[blank_end] organisms
b/ allows stain to be [blank_start]absorbed[blank_end]- Akill
- Babsorbed
38Each bacteria has two names and they define its
- Afamily and genus
- Bfamily and kingdom
- Cgenus and species
39Bacteria cell is
- Aeukaryotic
- Bprokaryotic
40Which of the following is NOT found in the cell wall of gram (-) bacteria?
- ALipid A
- BTeichoic acids
- CO-antigen
- Dpeptidoglycan
41Name the different types of bacteria pili and what are their function
a/ [blank_start]common[blank_end] and its function is [blank_start]adhesion[blank_end]
b/ [blank_start]sex[blank_end] and its function is [blank_start]conjugation[blank_end]- Acommon
- Badhesion
- Csex
- Dconjugation
42Loeffler stain requires
- AMethylene blue
- BZiehl fuschin
- CGentian violet
- DIodine solution
43Which of the following methods of staining are complex
- APfeiffer
- BNeisser
- CKlett
- DLoeffler
44Bacterium Diphteri and its structure : [blank_start]Volutine granule[blank_end]
- AVolutine granule
45The volutine granules of Bacterium Diphteri are visualized by : [blank_start]Neisser[blank_end] staining
- ANeisser
46The following structures are localized in the cytoplasm:
- ASpores
- BNucleoids
- CFlagellum
- DCapsules
47Based on their cellular structure bacteria are divided into:
- ACocci and rods
- BGram + and Gram –
- CSpares and spiral
- DAerobes and anaerobe
48Peptides Glycan polymer are found only in :
- ABacterial cell
- BAnimal cell
- CIn viruses
49Chemical structure of the capsule is composed of :
- AComplex polysaccharides
- BVitamins
- CPolypeptides
50Sporulation appears to be :
- AAn initiation of reproduction of bacterial cell
- BA reaction of conditions that are unfavorable for normal growth
- CA status to resist desiccation and other harmful conditions
- Da & b
- Eb & c
51The complete killing or removal of all microorganisms including bacteria spores is called :
- ASterilization
- BDisinfection
- CAntisepsis
- DSepsis
52What is the color of the simple method of Pfeiffer staining : [blank_start]red[blank_end]
- Ared
53Pfeiffer technical staining makes use of the following :
- AMethylene blue
- BCarbon Fuchsine
- CNeutral red
- DGentian violet
54The cell wall of gram (+) bacteria is composed mainly :
- AHitine (typical for plants)
- BPeptidoglycan (90%)
- CCellulose
- DSugars
55Which of the following bacteria have peptidoglycan layer:
- AGram (+) cocci
- BGram (-) bacteria
- CBoth of them
- DNone above
56Cellular DNA is concentrated in (what place) :
- ACytoplasm as a nucleoid
- BMitochondria
- CPlasmids as an extra chromosomal genetic material
- DRibosomes
57The spores stain by Moller are :
- ABlue color
- BRed color
- CBlack color
- DYellow color
58Which structure is NOT protecting bacteria from phagocytosis from leukocytes:
- ASpores
- BCapsule
- CFlagella
- DRibosome
59Name two simple (basic) stains
a/ [blank_start]Loeffler[blank_end]
b/ [blank_start]Pfeiffer[blank_end]- ALoeffler
- BPfeiffer
60The concentration of ethyl alcohol as an antiseptic for disinfection is :
- A95%
- B70%
- C50%
- D30%
61Bacterial nucleoid consists of :
- ANo chromosomes
- BOnly one chromosome
- CSeveral chromosomes
62What is the name of staining procedure for bacterial capsule:
- AGram stain
- BLoeffler stain
- CKlett stain
- DMoller stain
63Lipopolysaccharide can be found in large amount :
- AGram (+)
- BGram (-)
- CBoth of them
- DNone of above
64Complex method for bacterial staining are :
- ALoeffler
- BPfeiffer
- CGram
- DZiehl-Neelsen
65Bacterial antibiotic resistance is frequently covered by :
- ATempered bacteriophage
- BR-plasmids
- CLipid bacteriophage
- DIntron
66What constitutes a bacterial culture :
a/ [blank_start]Solute[blank_end]
b/ dry [blank_start]agar[blank_end]
c/ [blank_start]broth[blank_end]- ASolute
- Bagar
- Cbroth
67Which one is a liquid medium for bacterial culture:
- ABlood agar
- BGlucose broth
- CBoth of them
- DNone above
68Anaerobes are bacteria that can tolerate high oxygen concentration
- ATrue
- BFalse
69What bacterial physiologist studies :
- AMorphology of bacteria
- BBacterial structure
- CBacterial growth
- DBacterial nutrient
7070% ethanol is :
- ASterilizing agent
- BAntiseptic
- CAntibiotic
- DNone is true
71Which of the following is most important structure related to microbial attachment to cells :
- AFlagellum
- BPlasmid
- CPeptidoglycan
- DOrdinary Pilli
72Which statement about genetic transfer among bacteria requires cell to cell contact:
- AProcess of conjugation
- BProcess of transduction
- CProcess of transcription
- DProcess of auto combination
- EProcess of transformation
73Which of the following related to endotoxin is not true:
- AEndotoxins are secreted from living cells
- BEndotoxins are part of cellular gram (-) bacteria
- CThey are secreted only when the cell is disrupted
- DThey can be linked to meningococcemia
- EThey are produced by gram(-) microorganisms
- FThey cause fever
74What cause (hint) to be pink :
- ACarbon Fuchsin
- BIodine solution
- CCrystal violet
- DNeisser A
75Gram (-) bacteria differ from Gram (+) ones because the posses :
- ALipopolysaccharide
- BPeptidoglycan
- COnly cytoplasmic membrane
- DLipoteichoic acid
76The auto-sterilisation requires the following :
- A1:30 hours at 160 C
- B134 C for 10-15 minutes
77The chlorine is :
- AAntiseptic
- BDisinfectant
- CChemical agent for sterilization
- DAll of above
78Concerning bacterial cell division everything is true except that :
- AAll bacteria double their numbers every 10 hours
- BBacterial generation times are influenced by environmental conditions
- CTwo bacterial cells following division are genetically identical and they are once
79How does high temperature cause cell death?
- AOxidative damage
- BHigh level of electrolytes
- CProtein denature
- DAll of above
80How are the actions of phenols and alcohols similar :
- ABoth denature proteins
- BBoth oxidize DNA
- CBoth inhibit nucleic acid synthesis
- DAll of above
81What is pasteurization :
- AA method that disinfects the instruments
- BSterilizes food
- CA method that can be used on skin and mucus
- DAll of above
82Bacterial flagella are :
- AComposed of pillin
- BA floppy structure made of flagellin
- CRigger structure made of flagellin
83Name the complex nutrient media :
- AGlucose broth
- BMid peptone broth
- CMid peptone agar
- DBlood agar
84Extra chromosomal genetic elements in bacteria are :
- AMesosomes
- BPhagosomes
- CTransposomes
- DBacteriophages ( or plasmids)
85What part of the bacterial cell is most involved with Gram staining and why?
Ans: [blank_start]Cell wall[blank_end]
Explanation: Gram [blank_start]positive[blank_end] bacteria have a [blank_start]thick[blank_end] peptidoglycan cell [blank_start]wall[blank_end] that holds the stain [blank_start]better[blank_end] than the [blank_start]thin[blank_end] peptidoglycan gram [blank_start]negative[blank_end] cell [blank_start]wall[blank_end]- Apositive
- Bthick
- Cwall
- Dbetter
- Ethin
- Fnegative
- Gwall
- HCell wall
86What is the purpose of heat during the acid-fast staining procedure?
The heat [blank_start]drives[blank_end] the stain [blank_start]into[blank_end] the cells- Adrives
- Binto
87Name two curved bacteria:
a. [blank_start]Vibrio Cholerae[blank_end]
b. [blank_start]Helicobacteria[blank_end]- AVibrio Cholerae
- BHelicobacteria
88Name the reagent used and state the purpose of each of the following
a/ mordant: Reagent- [blank_start]Iodine[blank_end] solution, Function: form a [blank_start]complex[blank_end] between the crystal violet and [blank_start]iodine[blank_end]
b/ primary stain: Reagent- [blank_start]Gentian Violet[blank_end], Function: increases the [blank_start]contrast[blank_end] of both gram-negative and gram-positive bacteria
c/ decolorizer: Reagent- [blank_start]Alcohol[blank_end], Function: [blank_start]dehydrates[blank_end] the peptidoglycan layer
d/ counterstain: Reagent- [blank_start]carbol fuschin[blank_end], Function: stains solution light [blank_start]red[blank_end] allowing Gram [blank_start]negative[blank_end] to be seen- AAlcohol
- BIodine
- Ccomplex
- Diodine
- EGentian Violet
- Fcontrast
- Gdehydrates
- Hcarbol fuschin
- Ired
- Jnegative
89Round bacteria in chains are named [blank_start]streptococci[blank_end]
- Astreptococci
90What suggestions about endotoxins are true?
a/ they are typical for [blank_start]Gram (-)[blank_end]
b/ they act [blank_start]non-specific[blank_end]
c/ their chemical structure is [blank_start]lipopolysaccarides[blank_end]
d/ they are heat [blank_start]stable[blank_end]- AGram (-)
- BGram (+)
- Cnon-specific
- Dspecific
- Elipopolysaccarides
- Fproteins
- Gstable
- Hlabile
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- Microbiology Test 1 2nd Year PMU
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- Med Student
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