Quiz
Cell Structure Intro
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Questions in this quiz (18)
1 • Cell Types
○ There are over [blank_start]300[blank_end] cell types distinguishable by structure, function and location
○ Use [blank_start]antibodies[blank_end] for staining in microscopy
• Cell Structure
○ Basic Components
§ Plasma Membrane
§ C[blank_start]ytoplasm[blank_end]
§ N[blank_start]ucleus[blank_end]
• Plasma Membrane and Components
○ L[blank_start]ipids[blank_end]
○ P[blank_start]roteins[blank_end]
○ C[blank_start]arbohydrates[blank_end]
Fluid mosaic model of Plasma Membrane- A300
- Bantibodies
- Cytoplasm
- Ducleus
- Eipids
- Froteins
- Garbohydrates
2 § Hydrophilic heads and hydrophobic tails
§ [blank_start]Phospholipids[blank_end] arranged in a bilayer
□ Fluid inside (cytoplasm) and outside of cell
§ Hydrophobic tails made of [blank_start]fatty acids[blank_end]
□ Tails have [blank_start]kinks[blank_end] and are [blank_start]unsaturated[blank_end], double-bonded
§ Cholesterol is another lipid component that is hydrophobic
□ Adds fluidity to its [blank_start]steroid[blank_end] structure
Depending on [blank_start]temperature[blank_end] cholesterol can change fluidity using [blank_start]lipid[blank_end] rafts where cholesterol make them more [blank_start]static[blank_end] and they use to transport receptors around; want proteins to stay near each other for reactions- APhospholipids
- Bfatty acids
- Ckinks
- Dunsaturated
- Esteroid
- Ftemperature
- Glipid
- Hstatic
3 § Integral Membrane Proteins
□ Can insert partially through
□ Inserted all the way through membrane are called [blank_start]transmembrane[blank_end] proteins
□ These can be both hydrophilic or hydrophobic ([blank_start]amphipathic[blank_end])
§ Peripheral Proteins
□ Can sit on intracellular or extracellular areas of membrane
□ These are generally [blank_start]hydrophilic[blank_end]
§ Carbohydrates
□ Can be attached to lipids (glycolipids or glycoproteins)
□ Always on extracellular surface of membrane
® Purposes: signaling, [blank_start]recognition[blank_end]
® Don't need to glycolipid types
® Phospholipids: Phosphatidylc[blank_start]holine[blank_end], phosphatidyls[blank_start]erine[blank_end], phosphatidyl[blank_start]inositol[blank_end], phosophatidyle[blank_start]thanolamine[blank_end]
□ Glycolipids
® Gluco[blank_start]cerebroside[blank_end]
[blank_start]Galactocerbroside[blank_end]- Atransmembrane
- Bhydrophilic
- Camphipathic
- Drecognition
- Ecerebroside
- FGalactocerbroside
- Gholine
- Herine
- Iinositol
- Jthanolamine
4 § Membrane Lipid Structure
□ Asymmetrical Distribution of Membrane Lipids
® Some are in the extracellular space and others are in the cytosol
◊ Extracellular:
} phosphatidylc[blank_start]holine[blank_end]
} Sphingom[blank_start]yelin[blank_end]
} Cholesterol
} Glyco[blank_start]lipid[blank_end]
◊ Cytosol
} Phosphatidyls[blank_start]erine[blank_end]
} Phosphatidyli[blank_start]nositol[blank_end]
} Phosphatidyle[blank_start]thanolamine[blank_end]
§ Membrane Proteins
□ [blank_start]Receptor[blank_end]; sit on outside binds to specific ligand, Ex: transferin and specific ligand
□ Ion Channels: selective [blank_start]permeable[blank_end]
□ Integral Proteins
Transmembrane Proteins- Aerine
- Bnositol
- Cthanolamine
- Dpermeable
- EReceptor
- Fholine
- Gyelin
- Hlipid
5 § Membrane Carbohydrates
□ [blank_start]Oligosaccharides[blank_end] of glycoproteins and glycolipids found only extracellular surface of membrane
§ Membrane Appearance
□ Brightfield Miccroscopy
® Cell boundaries typically visualized through staining properties of membrane-associated [blank_start]carbohydrates[blank_end]
□ Scanning Electron Microscopy
® Cell boundaries can be visualized through deposition of heavy [blank_start]metals[blank_end] on membrane surfaces
□ Transmission Electron Microscopy
® Cell boundaries visualized through deposition of heavy metals (ie osmium)
® Really [blank_start]thin[blank_end] sections of tissue
□ Plasma Membrane
The plasma membrane and all intracellular membranes have a similar ([blank_start]trilaminar[blank_end]) appearance and similar biochemical composition (lipid bilayer)- AOligosaccharides
- Bcarbohydrates
- Cmetals
- Dthin
- Etrilaminar
6 □ Membrane Function
® Selectively [blank_start]Permeability[blank_end]
◊ Molecular passage across the hydrophobic region of the membrane-lipid bilayer
} Passive Transport:
– [blank_start]Simple[blank_end] Diffusion (ex. Aquaporin-integral protein water channels)
w Gases can also pass
– [blank_start]Facilitated[blank_end] Diffusion (ex. ion channels, binding, may need a protein)
} Active Transport
– [blank_start]ATP[blank_end] used here
◊ Processes that allow cross of this membrane
Movement of molecules down concentration gradient- AFacilitated
- BSimple
- CPermeability
- DATP
7 ® Endocytosis
◊ Phagocytosis (cell [blank_start]eating[blank_end]); membrane of cell surrounds particle ie. White [blank_start]blood[blank_end] cells, a[blank_start]moeba[blank_end]
◊ Pinocytosis (cell [blank_start]drinking[blank_end]) i.e occurs in ca[blank_start]pillaries[blank_end]
◊ Receptor-Mediated Endocytosis
Need a specific receptor to bind to ligand as well as a host of other proteins i.e clathrin on [blank_start]vesicle[blank_end] to enter cell- Aeating
- Bblood
- Cmoeba
- Ddrinking
- Epillaries
- Fvesicle
8 § Mitochondria
□ Electron transport occurs in the [blank_start]cristae[blank_end]
□ Glycolysis occurs in the [blank_start]matrix[blank_end]
□ Mitochondria have DNA and [blank_start]ribosomes[blank_end]
□ Most cells contain mitochondria with shelf-like cristae
□ Steroid-secreting cells contain mitochondria with tubular or vesicular cristae (ovaries, testes, adrenal)
□ Provide energy for mechanical [blank_start]energy[blank_end] for storing energy from cellular metabolites in high energy bonds of [blank_start]ATP[blank_end]
□ Citric Acid cycle uses acetyl [blank_start]CoA[blank_end] or fatty acids to generate high energy to drive and eject protons
□ Proton imbalance causes [blank_start]electrochemical[blank_end] gradient
Proton imbalance causes flow of [blank_start]protons[blank_end] down the gradient using ATP [blank_start]synthase[blank_end]- Acristae
- Bmatrix
- Cribosomes
- Denergy
- EATP
- FCoA
- Gprotons
- Hsynthase
- Ielectrochemical
9 § Endosymbiotic Theory
□ In this theory, the first eukaryotic cell was probably an amoeba-like cell that got nutrients by phagocytosis and contained a [blank_start]nucleus[blank_end] that formed when a piece of the cytoplasmic membrane pinched off around the chromosomes.
□ Some of these amoeba-like organisms ingested prokaryotic cells that then survived within the organism and developed a symbiotic relationship.
□ Mitochondria formed when bacteria capable of aerobic respiration were ingested; [blank_start]chloroplasts[blank_end] formed when photosynthetic bacteria were ingested.
□ They eventually lost their cell wall and much of their DNA because they were not of benefit within the host cell. Mitochondria and chloroplasts cannot grow outside their host cell.
□ Mitochondria can arise only from [blank_start]preexisting[blank_end] mitochondria. The eukaryotic nuclear genes encode only some of the proteins of which they are made.
□ Both mitochondria and chloroplasts have their own genome .
□ Both genomes consist of a single [blank_start]circular[blank_end] molecule of DNA.
□ Both mitochondria and chloroplasts have their own protein-synthesizing machinery, and it more closely resembles that of bacteria.
□ The first amino acid of their transcripts is always [blank_start]fMet[blank_end] as it is in
□ A number of antibiotics (e.g., streptomycin) that act by blocking protein synthesis in bacteria also block protein synthesis within mitochondria and chloroplasts.
The antibiotic rifampicin, which inhibits the RNA polymerase of bacteria, also inhibits the RNA polymerase within mitochondria- Anucleus
- Bchloroplasts
- Cpreexisting
- Dcircular
- EfMet
10 § Outer Mitochondrial Membrane Components
□ [blank_start]Porins[blank_end]
§ Inner Mitochondrial Membrane Components
□ Electron-Transport Chain
□ [blank_start]Cytochrome[blank_end] C
□ Mitochondrial ATP [blank_start]Synthase[blank_end] (F1 Subunits, [blank_start]Lollipops[blank_end])
§ Matrix Components
□ Tricarboxylic Acid (Kreb’s) Cycle [blank_start]Enzymes[blank_end]
□ Mitochondrial (Circular) [blank_start]DNA[blank_end]
□ Mitochondrial [blank_start]Ribosomes[blank_end]
Matrix [blank_start]Granules[blank_end] (Ca++)- AGranules
- BRibosomes
- CDNA
- DEnzymes
- ESynthase
- FLollipops
- GCytochrome
- HPorins
11 § Endoplasmic Reticulum
(RER) Synthesis of proteins destined for:
1. Se[blank_start]cretion[blank_end]
2. [blank_start]Isolation[blank_end] from the cytosol
3. [blank_start]Insertion[blank_end] into membranes
Other cellular proteins are synthesized on free polyribosomes
RER Function
1. mRNA for proteins to be secreted
or isolated contains an initial coding
sequence for a signal sequence
of [blank_start]hydrophobic[blank_end] amino acids
2. [blank_start]mRNA[blank_end] associates with
free polyribosomes
3. Ribosome translates [blank_start]signal sequence[blank_end] first
4. Signal sequence [blank_start]protrudes[blank_end]
from ribosome
5. Signal recognition particle (SRP)
[blank_start]binds[blank_end] to signal sequence and halts
translation
6. SRP Receptor (ribophorin) in RER
membrane recognizes and binds SRP/
signal sequence/ribosome complex
7. Binding of complex [blank_start]releases[blank_end] SRP
8. Translation resumes and nascent
polypeptide enters lumen of [blank_start]RER[blank_end] as
translation continues
9. Signal [blank_start]peptidase[blank_end] in RER lumen cleaves
signal sequence from nascent protein
10. Translation continues to completion
11. Ribosome detaches for reuse- Acretion
- BIsolation
- CInsertion
- DmRNA
- Ehydrophobic
- Fsignal sequence
- Gprotrudes
- Hbinds
- Ireleases
- JRER
- Kpeptidase
12 □ Core Glycosylation of sequestered proteins in RER lumen
□ Oligosaccharide chain elongation in [blank_start]Golgi[blank_end] Complex
□ Cell Types of Rich in RER
1. Protein-Secreting Cells
a. [blank_start]Plasma[blank_end] Cells
b. H[blank_start]epatocytes[blank_end]
c. Pa[blank_start]ncreatic[blank_end] [blank_start]Acinar[blank_end] Cells
d. F[blank_start]ibroblasts[blank_end]
e. C[blank_start]hondroblasts[blank_end]
f. O[blank_start]steoblasts[blank_end]
3. [blank_start]Protein[blank_end]-Storing Cells
a. M[blank_start]acrophages[blank_end]
b. [blank_start]Osteo[blank_end]clasts- APlasma
- Bepatocytes
- CAcinar
- Dncreatic
- Eibroblasts
- Fhondroblasts
- Gsteoblasts
- Hacrophages
- IProtein
- JOsteo
- KGolgi
13 § SER Function
1) Synthesis of [blank_start]Steroids[blank_end]
2) [blank_start]Lipid[blank_end] Metabolism
3) [blank_start]Glycogen[blank_end] Synthesis
4) [blank_start]Drug[blank_end] Detoxification
5) [blank_start]Ca[blank_end]++ Sequestration & Release (found in skeletal muscle)
□ Cell Types Rich in SER
1. Steroid-Secreting Cells
a. [blank_start]Adrenal[blank_end] Cortical Cells
b. [blank_start]Leydig[blank_end] Cells (Testis)
c. [blank_start]Granulosa[blank_end] Cells (Ovary)
2. Active Metabolic Cells
a. H[blank_start]epatocytes[blank_end]
b. [blank_start]Adipo[blank_end]cytes
3. [blank_start]Muscle[blank_end] Cells
a. [blank_start]Skeletal[blank_end] Muscle
b. C[blank_start]ardiac[blank_end] Muscle- ASteroids
- BLipid
- CGlycogen
- DDrug
- ELeydig
- FGranulosa
- GAdipo
- HMuscle
- ISkeletal
- Jardiac
- Kepatocytes
- LAdrenal
- MCa
14 □ Golgi Compartments:
® Flattened cisternae
® Small vesicles
® Few large condensing [blank_start]vacuoles[blank_end] at concave surface of stack
□ Golgi Faces
® [blank_start]Cis[blank_end] face, convex face
◊ Usually close to adjacent dilated ER cisternae & surrounded by transfer vesicles
® Trans face, [blank_start]concave[blank_end] face
◊ Harbors several condensing vacuoles & face [blank_start]away[blank_end] from nucleus
Connects system of tubule called [blank_start]TGN[blank_end] which secretory vesicles and lysosomes exit- Aconcave
- BCis
- Cvacuoles
- Daway
- ETGN
15 □ Golgi Complex Function
® Packaging of Sequestered Proteins and Other Secretory Products
® Glyc[blank_start]osylation[blank_end] of Proteins and Other Cell Products
® Sul[blank_start]fation[blank_end] of Proteins and Other Cell Products
® Vesicle Formation for Transport and Secretion
® Targeting of vesicles for intracellular transport
® Synthesizes secretory glycop[blank_start]roteins[blank_end], proteoglycans, gly[blank_start]colipids[blank_end] and [blank_start]sulfated[blank_end] GAGs
□ Packaging of Sequestered Proteins and Other Secretory Products
® Products of RER and other membranous organelles delivered to [blank_start]cis[blank_end] and medial Golgi by [blank_start]transfer[blank_end] vesicles
Modified and packaged by Golgi and released from medial and [blank_start]trans[blank_end] Golgi as transfer or [blank_start]secretory[blank_end] vesicles- Asulfated
- Bcis
- Ctrans
- Dsecretory
- Etransfer
- Froteins
- Gcolipids
- Hosylation
- Ifation
16 □ Vesicle Formation and Targeting
® Coat Proteins: Assist vesicle formation and [blank_start]targeting[blank_end]
◊ [blank_start]Clathrin[blank_end] (to and from plasma membrane)
◊ COP I ([blank_start]return[blank_end] processing proteins to organelles)
COP [blank_start]II[blank_end] (ER to Golgi - proteins being processed)- Areturn
- BII
- Ctargeting
- DClathrin
17 § SNAREs: Assist vesicle targeting
□ Lock & Key binding of vesicles to targets
® v-SNAREs (targeting protein in vesicle membrane)
◊ Critical for recognizing, binding & fusing with & unloading their contents in [blank_start]GTP[blank_end] dependent process
® t-SNAREs (v-SNARE receptor in target membrane)
Selectively bind and v-SNARE & recruit [blank_start]cytoplasmic[blank_end] proteins required for fusion with the target membrane- AGTP
- Bcytoplasmic
18 □ Cell Types Rich in Golgi Complex
1. [blank_start]Protein[blank_end] Secreting Cells
a. [blank_start]Plasma[blank_end] Cells
b. Hep[blank_start]atocytes[blank_end]
c. Pancreatic [blank_start]Acinar[blank_end] Cells
2. Matrix-Secreting Cells
a. Fibro[blank_start]blasts[blank_end]
b. Cho[blank_start]ndroblasts[blank_end]
c. Osteo[blank_start]blasts[blank_end]
3. Protein-Storing Cells
a. M[blank_start]acrophages[blank_end]
b. Oste[blank_start]oclasts[blank_end]- Androblasts
- Bblasts
- Cacrophages
- Doclasts
- Eblasts
- FAcinar
- Gatocytes
- HPlasma
- IProtein
Information
- Description
- Human Biology Quiz on Cell Structure Intro, created by Fatima G. on 30/08/2019.
- Author
- Fatima G.
- Language
- English
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