Fichas
Clinical pathology - sample collection + processing
Fichas del conjunto (16)
- 1What is a red top tube and what is it used for?Plain/serum tube
Biochemistry, bile acids, serology, endocrinology
Culture - 2What is a green top tube used for? What is a limitation to it?Heparin
Plasma biochemistry, avian/reptile haematology
May interfere with stains - 3What are blue top tubes used for? What will happen if the tube is under-filled?Sodium citrate
Fibrinogen tests, clotting times
Clotting times artifactually prolonged - 4What are purple top tubes used for? What occurs if the tube is underfilled and overfilled?EDTA - haematology, PCV, cytology
Underfilled - cell crenation, altered MCV/MCHC, reduced PCV, high refractometer result
Overfilled - microclot formation - 5What are grey top tubes used for?Potassium oxalate or sodium fluoride
Glucose - 6What are the differences between collected serum and plasma?Serum = plain-top tube; need 2.5x more whole blood than serum vol needed
- Must allow to clot, then centrifuge - 7What are standards for sample handling?Esky + icepack storage, wrapped in paper to avoid cell lysis
eat = macrocytosis and cell lysis
Special containers for blood smears
Make blood smears immediately - 8What aspects are important when collecting blood?Site of collection
Needle/syringe size
Vacutainer use
Vacuum applied
Anticogulant mixing + sequence of collection - 9What are some downfalls for needle and syringe collection?Potentially too much pressure applied
Needle too large/small
Haemolysis if emptied via needle
May clot inside needle - 10Why are vacutainers useful?Applies enough pressure for volume needed
Appropriate needle size
No clotting in needle - 11What does haemolysis do to a sample?Alters spectophotometer measurements
Invalidates erythrocyte results - 12What needle size would you use for a dog, cat, horse, and cow?Dog - 22 G
Cat - 22/23 G
Horse - 18-20 G
Cow - 20 G - 13Why should you minimise the number of "sticks" per collection site?Thromboplastin release from traumatised BVs will activate platelets
--> Microclot formation in sample - 14What is a haemogram tsunami?Microclot formation:
Not disconnecting the vacutainer before pulling the needle out
Unclean collection - 15What is an appropriate order of collection?Red top > citrate > heparin > EDTA > sodium fluoride
Citrate requires first bit of blood discarded (thromboplastin)
Prevents anticoagulant contamination of serum tube - 16How could you prepare a patient for blood collection?Fasting for 12 hours
--> Minimises lipaemia and consequential haemolysis
Información
- Descripción
- (Small animal veterinary medicine) Veterinary Medicine Fichas sobre Clinical pathology - sample collection + processing, creado por Alinta Kalns el 02/06/2018.
- Creado por
- Alinta Kalns
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- Valoración