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- 1DNA polymerase III has exonuclease activity - what is this?Exonuclease acts as a DNA proof reader which recognises mis-matched base pairs & removes offending nucleotides
- 2What role does DNA polymerase I perform?Contains 5' to 3' exonuclease activity that removes RNA primers before filling in the gaps left by RNA on the lagging strands
- 3What is the Central Dogma of Biology?1. REPLICATION (creation of duplex DNA from existing strands in the nucleus)
2. TRANSCRIPTION (Info in new DNA transferred to newly assembled mRNA in the nucleus)
3. TRANSLATION (genic info made into primary sequence of protiens by ribosomes - in cytoplasm) - 4What are Okazaki fragments?Short DNA moluecules found on the lagging strand
- 5What is a Nucleoid?Bundle of DNA found in bacterial cells
- 6What is a plasmid?A DNA molecule that is seperate from, and can replicate independantly of the chromosomal DNA
- 7What is a replicon?A DNA molecule/region of a DNA molecule that replicates from a single origin of replication
- 8What are the functionf of; DnaA, DnaB (Helicase), DnaC and DnaG (Primase)?DnaA = Binds to the ori & exposes individual DNA strands
DnaB = Unwinds dsDNA - powered by ATP
DnaC = Helps load Helicase onto DNA
DnaG = Synthesizes complementary short RNA primers from ssDNA in 5' to 3' direction - 9What is the process of assembly for DNA?1. dnaA binds to ori & opens up DNA
2. 2 helicase proteins are loaded on to the strand in opp. directions
3. dnaC helps load helicase
4. Primase added to start replication by synthesizing short complimentary RNA sequence
5. DNA Polymerase 3 binds & replicates DNA
6. Sliding clamp (dnaN) stops DNA polymerase from falling off
7. ss binding proteins prevent ssDNA from binding back together (annealing)
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- DNA Replication Fichas sobre Untitled, creado por olivia_barraclough el 04/05/2013.
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