Mapa mental
zygote
Contenido del mapa
zygote
Cloning possibilities
- ES cell transcriptome
- ESTs (expressed sequence tag)
- SAGE
- Expression microarrays
- MPSS (massively parallel signature sequencing)
- beads
- nuclear transfer (SCNT)
- cell from 8-cell into enucleated egg (ntES)
- using differentiated somatic cell
- reprogramming
- reprogramming capacity depends on cell-cycle status
- replace interphase nuc with somatic chromatin
- good
- MII-arrested oocyte
- zygote arrested with a drug in the first mitosis
- fail
- germinal-vesicle stage oocyte
- pronuclear zygote
- good
- replace interphase nuc with somatic chromatin
- cell fusion
- Tetraploid ES Cell
- MAS (multipotent adult spermatagonial) cell
- Tetraploid ES Cell
- Cell explanation
- spindle transfer
- mitochondrial replacement therapy
- Transdifferentiation = direct reprogramming
- transcription factors
- MicroRNAs
- small moleucules
- ES cell transcriptome
Stem cell
- Culturing
- embryoid body
- Feeder cell layer --> LIF +BMP
- 3D environment fibroblast feeder layer proconditioned by growth of teratocarcinoma cells
- special
- homologous proteins
- embryonal carcinoma cell lines
- Cell memory
- cisplatin
- epigenetic regulation/modifications
- histone
- Nucleosome
- histone tails
- Phosphorylation
- methylation
- acetyalation
- DNA
- histone deaceylation
- microRNA (miRNA)
- silent (condensed) chromatin
- histone
- signaling
- combinatorial signaling
- cell memory
- x-inactivation
- Barr body of one of two x chromosome
- XIST and XACT
- x-inactivation
- types
- pluripotent
- regulators
- Oct4
- Sox2
- Nanog
- iPS
- Oct4, Sox2, KII4, c-Myc
- Source
- skin cells
- keratinocyte (hair cells)
- host
- blastocyst-stage embryos
- org
- chimeric
- derived cells
- 3 derms + germ cells
- limit
- low eff
- uses virus
- criteria for pluripotency
- assays
- totipotent, naive pluripotent, primed pluripotent, and multipotent developmental potentials.
- blastocyst chmaerism
- teratoma formation
- A huge number of in vivo assays are restricted or prohibited especially single-cell in vivo assays
- forensic genomics
- transcriptome analysis
- epigenomic characterization
- whole genome sequencing
- Injection of diploid pluripotent stem cells with ICM-like properties
- pre-committed ICM
- contributing primarily to the ICM = structures of the postnatal body
- committed\formed ICM
- postnatal body derived both from the ICM and from the stem cells.
- Cells that are 100% TE
- contributing primarily to the ICM = structures of the postnatal body
- pre-committed ICM
- developmental potential
- in vitro differentiation
- Teratoma
- resetting to ground state PS cell: prime --> metastable PS cell --> ground state
- assays
- regulators
- multipotent
- adult (somatic) stem cells
- Unipotent
- totipotent
- pluripotent
- ES
- accessbile chromatin
- increased global transcription
- gene expression
- splicing isoforms
- intergenic spliced mRNAs
- non-coding intergenic transcipts
- antisense transcripts
- increased global transcription
- accessbile chromatin
- epigenetics
- oocyte-to-embryo transition
- division schemes result in different cells
- asymmetric division
- symmetric division renewal
- symmetric SC commitment (differentiation)
- distribution of ER and mitochondria
- demethylation of pat and mat genomes
- 4-cell = zygotic genome activation
- mat mRNA degrade
- de novo transcription from embryonic genome
- division schemes result in different cells
- Embryonal carcinoma cells (EC)
- Culturing
2-cell
- 4-cell
- Morula
- Vertebrate embryos = blastocyte
- Inner cell mass
- Primitive ectoderm
- gastrulation
- Germ layers
- primitive/endoderm
- Pancreatic cell
- lung cell
- XEN (extraembryonic endoderm) cell
- ectoderm
- Nerve cell
- Skin cell
- Mesoderm
- Skeletal muscle cell
- Blood cell
- Hematopoietic stem cell
- detection assays
- in vitro
- LTC-IC
- maintenance of progenitors for 5 to 7 weeks
- CAFC
- growth as "cobblestones" for 5- 7 weeks
- LTC-IC
- in vivo
- CFU-S
- long term reconstitution
- most stringent
- NOD/SCID
- immunocompromised mice
- distinguish host and donor cells
- PCR-marker
- Chromosomal markers
- Enzyme markers
- proliferation assay
- Label retention assay
- in vitro
- enrichment and purificaiton
- Fluorescence Activated Cell Sorting (FACS)
- column-based magnetic enrichment
- redefined model
- FACS
- developmental shift in the progenitor cell architecture resulting in a two-tier hierarchy by adulthood.
- RNA-seq
- mixed-lineage transition states
- Combined loss of these two determinants ‘trapped’ the metastable intermediate. We propose that mixed-lineage states are obligatory during cell fate specification, manifest differing frequencies because of their dynamic instability and are dictated by counteracting gene regulatory networks.
- mixed-lineage transition states
- FACS
- detection assays
- Epi (epiblast) - 5.5 day
- primitive/endoderm
- Germ layers
- primordial germ cells
- He's like really nailing on some concepts take note of that
- germ cells/line
- properties
- high dev capacity
- disrupted = teratocacinomas
- gastrulation
- Primitive endoderm
- ES (embryonic stem) cell - 3.5 day
- human vs mouse
- development slower than mouse
- epigentic marks similar
- mouse
- activin/nodal
- human
- Naive
- ICM-like
- primed
- Epiblast-like
- Naive
- human vs mouse
- Primitive ectoderm
- Trophectoderm
- mural trophectoderm
- Polar trophectoderm
- Extra-embryonic ectoderm
- TS (trophoblast stem) cell
- Inner cell mass
- Vertebrate embryos = blastocyte
- Morula
- 4-cell
HOX genes
Información
- Descripción
- Biology Mapa Mental sobre zygote, creado por Trung Hoang el 26/02/2019.
- Creado por
- Trung Hoang
- Visitas
- 0
- Valoración