Gen. Lec. 11/1 (Chapter 13; Prokaryotes)

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All about those prokaryotes.
Isabella N
Flashcards by Isabella N, updated more than 1 year ago
Isabella N
Created by Isabella N over 6 years ago
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Question Answer
Bacteria has no ___ for transcription to happen in. Organelle
Lagging + leading strands are made @___ time by ___ _____. Same; DNA polyermase
DNA polymerase moves in (1) direction. 5' to 3'.
Transcription is done by who + makes what? RNA polymerase; a single-strand of RNA copy of a gene
mRNa is the "____ man" between DNa becoming protein. Middle
RNA polymerase makes ____ w/o a ___ ____. mRNA; RNA primer.
RNA polymerase uses...? ribonucleotides,
RNA is based on a ___ _____. DNA template
(3) phases of transcription Intitation, elongation, termination
Initiation regulates the amount of...? protein is made from mRNA.
Prokary. have a ___ polymerase. RNA
RNA polymerase moving upstream is...? 3' to 5'
RNA polymerase moving downstream..? 5' to 3'.
Ligase is used to created the missing...? Phosphodiester bond between nucleotides.
The promotor's job is to...? Specify where transcription begins.
In prokar., the promoter's name (sequence). -10, -35 region on the transcribed DNA.
The binding of RNA polymerase + promotor is done when? During intiation
DNA coming undone is done when? Initiation.
Link phosphodiester bonds between 1st (2) nucleotide @ 5' end of RNA. Initiation
The RNA is complete when? Elongation
The termination stage ends when...? The signal is given by a certain nucleotide sequence.
In prokar., their RNA polymerase has a _ factor to help polymerase to...? sigma (σ); find the promotor
The σ facter leaves polymerase after...? Polymerase binds to the promoter region + makes 8-9 nucleotide for the RNA strand.
Transcription happens ____ ____ a long the ____ strand of DNA. many times; same
Each promoter along the same strand of DNA is responsible for...? Their own section of mRNA to make
The polymerase finishes transcription when reaches the...? Terminator region
What is the termination signal to the polymerase? A hairpin sticks out of the mRNA is makes.
At the termination region of DNA, the RNA polymerase...? Comes off DNA strand.
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