Flashcards
API 20E results and interpretations
Cards in this deck (14)
- 1ONPG testBeta galactosidase digests ONPG to give ortho-nitrophenol
Colourless --> yellow - 2ADHArginine --> citruline + NH3
pH increase
Yellow --> red/orange
Phenol red indicator - 3LDCLysine --> cadaverine
pH increase
Yellow --> red/orange
Phenol red indicator - 4ODCOrnithine --> putriscine
pH increase
Yellow --> red/orange
Phenol red indicator - 5CITCitrate --> alkaline ammonium salt
pH increase
Bromothymol blue indicator --> blue - 6H2SBlack deposit/thin line as ferrous sulfide produced
- 7UREDegrades urea --> NH3
Increases pH
Yellow --> orange/red - 8TDAYellow --> dark red-brown
Ferric hydrazone produced - 9INDKovac's reagent turns pink if indole (product of 1st reaction) is present
- 10VPAcetoin reacts w/ VP1, VP2, and creatine to give red colour
- 11GELGelatinase degrades gelatine into a liquid, causing the black pigment (India ink) to diffuse
- 12OthersFermentation/oxidation
Blue --> yellow - 13OXCytochrome oxidase oxidises cytochrome, releasing...
- 14—
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- Year 2 BIO258 - Microbes and Man Flashcards on API 20E results and interpretations, created by Sophie Byrne on 14/11/2018.
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- Sophie Byrne
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