16012491
Description
Flashcards by Sophie Byrne, updated more than 1 year ago
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Created by Sophie Byrne
over 5 years ago
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| Question | Answer |
| ONPG test | Beta galactosidase digests ONPG to give ortho-nitrophenol Colourless --> yellow |
| ADH | Arginine --> citruline + NH3 pH increase Yellow --> red/orange Phenol red indicator |
| LDC | Lysine --> cadaverine pH increase Yellow --> red/orange Phenol red indicator |
| ODC | Ornithine --> putriscine pH increase Yellow --> red/orange Phenol red indicator |
| CIT | Citrate --> alkaline ammonium salt pH increase Bromothymol blue indicator --> blue |
| H2S | Black deposit/thin line as ferrous sulfide produced |
| URE | Degrades urea --> NH3 Increases pH Yellow --> orange/red |
| TDA | Yellow --> dark red-brown Ferric hydrazone produced |
| IND | Kovac's reagent turns pink if indole (product of 1st reaction) is present |
| VP | Acetoin reacts w/ VP1, VP2, and creatine to give red colour |
| GEL | Gelatinase degrades gelatine into a liquid, causing the black pigment (India ink) to diffuse |
| Others | Fermentation/oxidation Blue --> yellow |
| OX | Cytochrome oxidase oxidises cytochrome, releasing... |
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