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Flashcards by Glen Rooney, updated more than 1 year ago
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Created by Glen Rooney
almost 3 years ago
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| Question | Answer |
| What are the energy sources and carbon sources of chemoautotrophs? | Energy Source - Inorganic Molecules or Ions Carbon Source - Carbon Dioxide Example - Some prokaryotes such as sulfolobus and methanogen |
| What are myosins? | Motor proteins that interact with actin filaments and couple hydrolysis of ATP to conformational changes. This results in the movement of myosin and an actin filament relative to each other. Best known for their roles in muscle contraction |
| What does ubiquitin do? | It 'labels' proteins for degrading by covalently attaching to it. The protein is then broken down by proteasomes. |
| What are microtubules made of? | A dimer of tubulin with one α-subunit and one β-subunit. ie they are heterodimers. |
| Define growth factors | General term for molecules that bind to appropriate receptors and ultimately alter gene expression to stimulate growth, survival or division of the cell. |
| Name the 3 main types of cell surface receptors and give an example of a ligand involved in their interaction. | 1. Ion channel-linked receptor (Ligand - Acetylcholine) 2. G protein-coupled receptor (GPCR) (Ligand - Adrenalin/Noradrenalin) 3. Enzyme-linked receptor (Ligand - Cytokines and growth factors) |
| Define aneuploidy | When cells have an abnormal number of chromosomes. This occurs when the kinetochore attaches to the wrong microtubule before anaphase. |
| What are the 4 key features of cell communication? | 1. Signal 2. Reception 3. Transduction 4. Response |
| What are the energy sources and carbon sources of photoautotrophs? | Energy source - Light Carbon source - Carbon dioxide Example - Photosynthetic prokaryotes such as cyanobacteria ; Plants ; Algae |
| Why would protons cross the inner mitochondrial membrane via an uncoupler rather than via ATP synthase? | Movement via the uncoupler is more energetically favourable as it doesn't entail synthesis of ATP. |
| What are dyneins? | A type of motor protein. Move toward the minus end of microtubule. Moves in the opposite direction of kinesin. Important in vesicle trafficking and localisation of Golgi Apparatus |
| What is an allosteric effector? | A ligand that binds to a specific site on a protein and thereby induces a conformational change in the protein at a different site. |
| What are the proteins that attach the extracellular matrix to the cell membrane? | Integrins |
| Name the 3 muscle cell types and their function | Smooth muscle of internal organs eg intestines (Function - Peristalsis) Cardiac muscles (Function- Heart contraction) Skeletal muscles (Function - Movement of limbs) |
| What is Rho-family GTPase? | A subgroup of the GTPase family. Its activation leads to reorganisation of the cytoskeleton and affects cell adhesion. eg By influencing interactions between integrins and the actin network |
| What do chaperones do? | Help large proteins to fold. Prevent inappropriate interactions. Helps fix misfolds. Stops polypeptide mixing with other molecules. |
| What are mitogens? | A specific class of cell signals that trigger cell division. They stimulate the transcription of the G1 cyclins. |
| How do retinoblastoma act as a break in the cell cycle? | It binds to and inactivates the transcription factors which promote expression of the genes that encode proteins needed for cell division. |
| What is an example and function of an adipocyte? What is the main component of its cytoplasm? | Adipose tissue around organs and under the skin. Function - energy storage. Protection. Has cytoplasm made mostly of lipid. |
| What is meant by amphipathic? | A molecule that has both hydrophilic and hydrophobic parts. |
| What is the microtubule organising centre (MTOC)? | Region of cytoplasm from which microtubules radiate. The MTOC determines the pattern and number of microtubules. Animal cells usually have one called the centrosome located near the nucleus. |
| Phosphorylation is catalysed by the enzyme ___________. Dephosphorylation is catalysed by the enzyme __________. | 1. Kinase 2. Phosphatase |
| If an energetically unfavourable reaction is coupled to an energetically favourable, the change in free energy (∆G) of the overall process must be _________ for it to occur. | Negative |
| What is chemotaxis and chemotropism? | Chemotaxis: Movement directed by chemical gradients Chemotropism: Growth directed by chemical gradients. |
| What is RME? | Receptor-mediated endocytosis. It is a way macromolecules and macromolecular complexes can transport across membranes. Specific cell surface receptors bind to the ligand, a pit is then formed which is then pinched off into the cytosol. |
| What are the 3 major roles of general transcription factors? | 1. Positioning the RNA polymerase correctly at the promotor. 2. Separation of the 2 strands of DNA to allow transcription to begin. 3. Release of RNA polymerase from the initiation complex to start transcription. |
| Polypeptides are synthesised in which direction? | From the amino (-NH2) or N-terminus, towards the carboxyl (-COOH) or C-terminus. |
| What is the energy source and carbon source of photoheterotrophs? | Energy source - Light Carbon source - Organic compounds in the diet Example - Some prokaryotes such as rhodobacter |
| Can ions be moved against their concentration gradient? | Yes, by using active transport and transporter proteins. |
| What is the difference between competitive and non-competitive inhibitors? | Competitive inhibitors compete with the substrate for the active site on the enzyme. Non-competitive inhibitors don't bind to the active site. Instead they bind to another area and changes the conformation of the active site. |
| What organelle packages and tags proteins for proper distribution in the cell? | The Golgi Apparatus |
| What is feedback inhibition? | It is a cellular control mechanism where the product of a pathway inhibits an enzyme that caused a reaction earlier in the pathway, therefore regulating how much end product is produced. |
| Define what is meant by a metabolic pathway. | A linked series of chemical reactions within the cell in which the product of one reaction becomes the substrate for another. |
| What are integrins? | Transmembrane receptors that facilitate cell-cell and cell-extracellular matrix adhesion. |
| What happens to the 5' and 3' ends of mature mRNA during post-transcriptional modification? | Capping occurs at the 5' end. Polyadenylation occurs at the 3' end. |
| What do proteasomes do? | Proteasomes are protein complexes which degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. Enzymes that help such reactions are called proteases. Helps regulate protein levels and levels of gene transcription/translation. |
| What is the energy source and carbon source of chemoheterotrophs? | Energy source - Organic compounds Carbon source - Organic compounds in the diet Example - Animals; fungi; protists and many prokaryotes |
| Synthesis of the RNA transcript occurs in which direction? | 5' to 3' |
| What are the 2 forms of cell division carried out by complex eukaryotes? | Mitosis - generates 2 daughter cells with the same number of chromosomes as the parent. Meiosis - produces haploid gametes with half as many chromosomes as the parent. |
| What is a motor protein? | A protein that binds to something else and uses energy from ATP hydrolysis to travel along a microtubule or microfilament, taking the cargo with them. |
| Are potassium levels higher inside or outside an animal cell? And is the membrane potential of a non-excited cell positive, negative or neutral? | In an animal cell potassium levels are much higher inside the cell. The membrane potential is negative (-70mV) in a non-excited cell. |
| What are sense strands and antisense strands? | Sense strands are the non-template strands used in transcription. Antisense strands are the template DNA strands. |
| What is the function of red blood cells? Name 2 features of red blood cells. | Function - Oxygen transport Features - RBCs have haemoglobin which binds to oxygen. Mammalian RBCs lose their nucleus when mature. |
| The sum of catabolism and anabolism is __________. | Metabolism |
| MCPs that bind a repellant are ________ while those that bind an attractant are __________. | 1. Activated 2. Inactivated (MCPs - Methyl-accepting chemotaxis proteins) |
| What are kinesins? | A type of motor protein. Involved in cytokinesis and in the trafficking of vesicles and organelles within the cell. Moves toward the plus end of the microtubule. Runs in the opposite direction of dyneins. |
| Give examples of support cells (connective tissue cells). What is their function? | Examples - Bone cells (osteoblasts / osteoclasts), cartilage, fibroblasts. Function - Provides support and organises tissue structure. |
| What is a fibroblast? | A fibroblast is a type of cell that synthesises the extracellular matrix and collagen, produces the structural framework (stroma) for animal tissues, and plays a critical role in wound healing. Fibroblasts are the most common cells of connective tissue in animals. |
| Define transcription factors | Proteins that bind to specific DNA sequences promoting (activators) or inhibiting (repressors) the recruitment of RNA polymerase to specific gene promotors, thereby controlling the transcription of genes. |
| RNA polymerase reads the base sequence of the template strand in which direction? | 3' to 5' |
| What are the stages (in order) of meiosis? | Prophase I, Metaphase I, Anaphase I, Telophase I, Cytokinesis Prophase II, Metaphase II, Anaphase II, Telophase II, Cytokinesis |
| What are the 2 main ways in which an enzyme can be controlled in metabolism? | 1. Regulating the amount of enzyme present by limiting its expression or rate of degradation. 2. Through allosteric regulation. |
| Name some examples of coenzymes and describe their function and key features . | Examples - ATP, Acetyl CoA, NAHD, FAD Small, organic energy carriers. Can be inactivated (low energy) or activated (high energy). Activated coenzymes stores energy and releases it for endergonic reactions. This happens by coupling the reaction with the conversion of the activated coenzyme into its inactive state. Some coenzymes are a source of chemical groups needed for reactions eg phosphate group from ATP. |
| What is an endergonic reaction? | An endergonic reaction (such as photosynthesis) is a reaction that requires energy to be driven. Endergonic reactions are nonspontaneous. The change of Gibbs free energy (ΔG) during an endergonic reaction is a positive value because energy is gained. |
| What is the difference between a nucleolus and a nucleoid? | Nucleolus - is found inside the nucleus of eukaryotes. Is made of RNA and proteins. It is best known as the site of ribosome biogenesis. Nucleoid - is only found in prokaryotes. Irregularly shaped region that houses genetic material. Is not surrounded by a nuclear membrane. |
| What are MCPs and what domains do they have? | Methyl-accepting chemotaxis proteins. Receptors involved in chemotactic regulation of flagellar movement in bacteria. Has a periplasmic domain which binds the ligand (attractant or repellant), a transmembrane domain and a cytosolic domain which interacts with a series of downstream signalling proteins called Che proteins. |
| What are the stages of mitosis in order? | Interphase Prophase Metaphase Anaphase Telophase (Cytokinesis happens after mitosis is complete) |
| What are the cells of the immune system? | Lymphocytes (T-cells, B-cells), neutrophils, and macrophages. These are all types of white blood cells. The major proteins of the immune system are predominantly signaling proteins (often called cytokines), antibodies, and complement proteins. |
| Where would you find epithelial cells and what is their function? | The epidermis; lining of the intestines, blood vessels and lungs; cells of glands Function - protection, barrier, absorption, secretion. |
| Examples, function and features of nerve cells (neurons). | Examples - Brain neurons and spinal cord neurons; small groups of neurons (gnaglia) in the body Function - Quick and specific communication Features - Polarised cells with long processes; Specialised membrane to allow electrical signalling |
| Examples, function and features of endocrine cells. | Example - Pancreas; adrenal glands. Function - Widespread communication. Feature - Produce and secrete chemical messengers into circulation. |
| What is endocytosis and exocytosis? | Endocytosis - A general term for uptake of minerals into the cell by invagination. If foreign particles are taken up it is referred to as phagocytosis. Exocytosis - The release of materials enclosed in a vesicle from the interior of the cell to the exterior. |
| What biomolecules are required for in vitro DNA synthesis in the polymerase chain reaction (PCR)? | A double-stranded template DNA DNA polymerase (to catalyse the addition of nucleotides to a new DNA strand) The four dNTPs (dATP, dTTP, dCTP, dGTP) Two oligonucleotides to act as primers (one for each strand) |
| What are oligonucleotides? | Short lengths of single-stranded DNA generally around 20 nucleotides long. Used in PCR as primers. |
| Why does DNA synthesis require oligonucleotides to act as primers in PCR? | DNA polymerase cannot initiate the synthesis of a DNA strand. Instead, polymerisation begins by addition of nucleotides to an existing short nucleic acid primer annealed to the template strand. |
| What are the 3 phases of each round of PCR and what temperatures do they occur? | 1. Denaturation (94°C) 2. Primer annealing (55-58°C) 3. Elongation (74°C) |
| What DNA polymerase is commonly used in PCR? | Taq DNA polymerase Derived from the bacterium Thermus aquaticus |
| What are restriction endonucleases? | Enzymes that catalyse the cleavage of isolated DNA molecules at specific sequences in vitro. |
| What processes in living cells require DNA ligase? | Joining Okazaki fragments during lagging-strand DNA synthesis, and several of the mechanisms that repair DNA |
| What is the link reaction and where does it take place? | The second stage in glucose oxidation by aerobic respiration is the link reaction, so-called because it links glycolysis and the tricarboxylic acid (TCA) cycle. It takes place in the mitochondrial matrix. |
| What are the names of these cell cycle checkpoints and what is monitored at each one? | |
| What is the role of p53 in the cell cycle? | p53 is a transcription factor that is activated by damage to the DNA. It halts progression through the cell cycle by, among other things, promoting the transcription of the cyclin-Cdk inhibitor p21 |
| What is peptidoglycan? | Major component of bacterial cell walls. It is a polymer of high molecular mass, composed of two complex monosaccharides derived from glucose, which are linked together by amino acids, including three amino acids that are not found naturally in proteins. |
| Give three examples of the shapes exhibited by different bacterial species, and name one species of bacterium that exhibits each shape. | |
| Name the three main types of tissue in flowering plants and state the functions of each of these types of tissue. | Ground tissue -packing and support, energy storage, photosynthesis Vascular tissue - transport of water and nutrients Dermal tissue - protection, control of uptake of water, nutrients and gases |
| Name the structures labelled A, B, C and D and state their functions. | |
| What is a nonsense mutation? | A mutation which introduces a stop codon into the middle of an open-reading frame. |
| What is a null mutation? | A mutation which results in a complete loss of function of the mutated gene. |
| What happens in a transition mutation? | One purine is replaced with another purine or one pyrimidine is replaced with another pyrimidine |
| What happens in a transversion mutation? | A purine is replaced with a pyrimidine or vice versa |
| What is a frameshift mutation? | A mutation that arises by deletions or insertions of nucleotides and changes the frame in which triplet codons are first transcribed into RNA and then translated into polypeptides |
| What are the different types of non-coding DNA found in the human genome? | Non-coding DNA includes: introns, gene regulatory sequences, repetitive DNA (including transposable elements and short repeats), and also unique (i.e. non-repetitive) non-coding sequences. Together these constitute about 99% of the human genome. |
| In eukaryotes there are two main groups of transcription factors. What are they? Give a specific example of each type. | In eukaryotes there are general transcription factors (required for RNA polymerase binding) and specific transcription factors (that regulate patterns of genes expression in a particular cell). TFIID is an example of a general transcription factor (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, TFIIJ are others); MyoD1 is a sequence-specific transcription factor (also GATA-1, Sp 1, Oct-1, heat shock factor, etc.). |
| Some proteins are synthesised as inactive precursors known as ________. | Zymogens. Zymogen activation involves the hydrolysis of specific peptide bonds by proteases, and can involve removal of a length of polypeptide chain. |
| What is the lipid raft hypothesis? | According to this hypothesis, membranes contain microdomains (rafts) which are highly ordered. Their particular lipid composition means that lipid rafts are more cohesive and less fluid than the rest of the membrane. Thus lipid rafts can be envisaged as ‘islands’ in a sea of fluid membrane. |
| What is the Tm of a cell membrane? | Transition temperature (Tm): temperature at which a membrane changes between the fluid and gelled state. |
| List at least five features of microtubules | Microtubules are hollow tubes (25 nm diameter) Unit is a dimer of the protein tubulin: one α-tubulin subunit and one β-tubulin subunit (hence these are heterodimers) Dimers are all orientated in the same direction Microtubule assembly favoured when subunits are bound to GTP Microtubules have polarity: the end with α-tubulin exposed is the minus (−) end and the end with β-tubulin exposed is the (+) end Microtubules originate at the microtubule organising centre (MTOC) In animal cells the MTOC is usually a single centrosome in which the microtubules are tethered by the minus (−) pole. |
| How is motor protein activity rendered essentially irreversible? | Activity is made essentially irreversible by the large free energy release on hydrolysis of the ATP, i.e. a high energy barrier is presented for the reverse reaction. |
| What are transposons? | Small genetic elements that can move, or transpose, from one position to another in a genome; usually by copying itself and inserting into a second site, but sometimes by splicing out of the original site and inserting in a new location. |
| What are the three types of protein filament that make up the cytoskeleton? What are their component proteins and what are their main functions? | |
| What are telomeres? | Regions at the ends of eukaryote chromosomes consisting of short non-coding nucleotide repeats. Their presence avoids the loss of coding regions during DNA replication (the 3' end of the lagging strand cannot be replicated all the way to its end). |
| What is the difference between immunocytochemistry and immunohistochemistry? | Immunohistochemistry is a staining technique that uses entire sections of tissue. Immunocytochemistry is a staining technique that stains individual layers of cells. |
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