Mitochondrial Targeting and Transport

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Flashcards by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312 over 7 years ago
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Structural Basis for Biological Function (Protein Translocation) Flashcards on Mitochondrial Targeting and Transport, created by gina_evans0312 on 12/21/2013.

Resource summary

Question Answer
Length of Mitochondria target sequence 10-60 residues
Residues in Mitochondria target sequence Alternate- +ve and hydrophobic
Structure of Mitochondrial Target Sequence Forms an ampipathic helix- may or may no be cleavable
Import Pore- TOM40 4-8 monomers that form the pore
Import Pore- TOM22 Receptor for unfolded protein, and rebinds polypeptide when it's passed the pore to prevent it aggregating
Import Pore- TOM20/70 Associated receptors
Translationally Coupled- TOM20 Recognizes ampipathic helix of unfolded protein
Translationally Coupled- Chain of proteins TOM20-TOM22-TOM5-TOM40
(Yeast) Post Translational- MSF Mitochondrial Import Stimulating Factor
MSF binds... Once protein has been folded by ribosomal (yeast) or cytoplasmic (mammals) chaperones
At pore, MSF interacts with TOM70, which recognises it
Carrier proteins Carry other molecules in and out of mitochondria, and also pass through pore
TIM23/27 Forms import pore
N-terminus of TIM23 Recognises MSF
TIM44 Recruits matrix Hsp70 (and Hsp40/NEF) to TIM complex to fold it as it enters the mitochondria
Lateral Gate Allows protein to enter mitochondrial matrix
Mitochondrial Processing Peptide Removes mitochondrial targeting factor (cleaves at penultimate residue)
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