Experimental Methods: Bio110-2016

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Mind Map by cara brand, updated more than 1 year ago
cara brand
Created by cara brand almost 4 years ago
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Workflow of experimental methods discussed in intro Bio

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Experimental Methods: Bio110-2016
1 Nucleotides
1.1 Do I want to know where they are made?
1.1.1 Yes
1.1.1.1 Pulse experiment... I know what radioactive precursor to use, right?
1.1.1.1.1 Autoradiography
1.1.1.1.1.1 Do I want to visualize the cell?
1.1.1.1.1.1.1 Microscopy... I know which kind I should be using, right? And how to prepare my cells or tissues?
1.1.1.1.1.1.1.1 Light or Flourescent Microscopy
1.1.1.1.1.1.1.2 Electron Microscopy
1.1.1.1.1.2 Do I want to differentiate between locations in the cell
1.1.1.1.1.2.1 Cell fractionization... I know which cell components separate at different speeds, right?
1.1.2 No, I want to know where they're going?
1.1.2.1 Pulse + Chase experiment
1.1.3 I just want to label them!
2 Proteins
2.1 Am I interested in a specific protein?
2.1.1 No, I'm interested in them all
2.1.2 Yes!
2.1.2.1 Well then I need an Antibody... I know how to make one, right?
2.1.2.1.1 Do I want to visualize where the protein localizes in the cell?
2.1.2.1.1.1 Fluorescent labeled antibody?
2.1.2.1.1.2 Gold labeled antibody?
2.1.2.1.2 Do I want to test which tissues, or individuals, or samples contain my protein?
2.1.2.1.2.1 Studying Tissues: Harvest Sample
2.1.2.1.2.2 Studying cell components: fractionization
2.1.2.1.2.2.1 Purify proteins. Use SDS
2.1.2.1.2.2.1.1 Gel Electrophoresis... before I visualize my protein of interest, I have to size separate all of them
2.1.2.1.2.2.1.1.1 Western Blot
2.1.2.1.2.3 Studying individuals: sample individuals
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