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Cyt C Oxidase & F1F0
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Questions in this quiz (41)
1What is the main role of cytochrome aa3?
- ATakes e- from ubiquinol and reduces oxygen to water
- BTakes e- from ubiquinol and reduces oxygen to peroxide
- CTakes e- from cytochrome c and reduces oxygen to peroxide
- DTakes e- from cytochrome c and reduces oxygen to water
2Complex IV contains how many subunits?
- A12
- B13
- C14
- D15
3Complex IV doesn't transfer protons across the inner mitochondrial membrane
- ATrue
- BFalse
4Which ions does it contain, and how many of each?
- A2 Fe (in haeme groups) and 3 Cu (2 as a Cu-Cu binuclear centre, 1 as a binuclear Fe-Cu centre)
- B2 Cu (in haeme groups) and 3 Fe (2 as a Fe-Fe binuclear centre, 1 as a binuclear Fe-Cu centre)
- C2 Mg (in haeme groups) and 3 Cu (2 as a Cu-Cu binuclear centre, 1 as a binuclear Fe-Mg centre)
5Cytochrome aa3 complex consists almost entirely of alpha helices
- ATrue
- BFalse
6Put the following in order
A- Cyt c
B- CuA centre
C- Cyt a
D- Cyt a3-CuB centre
E - Water
•Transfer from cyt a3-CuB centre to oxygen- AA-B-C-D-E
- BA-C-D-B-E
- CA-C-B-D-E
7How many protons are pumped by complex 4, and how many are used to make water (per Oxygen molecule)?
- A4 & 4
- B4 & 2
- C2 & 4
8Which conformation of Complex IV has the low affinity for protons?
- AConf. A
- BConf. B
- CConf. C
9Protons bind to Complex IV during Conformation B
- ATrue
- BFalse
10What happens during conformation B that lowers the Complex IV's affinity for protons?
- AEnergy is put in
- BEnergy is lost
- CThe protein has an Fe group added
11No question, just look at it and learn it
- AOkay?
- BDone
12ATP synthase is found on the inner mitochondrial membrane
- ATrue
- BFalse
13ATP synthase is divided into two sections, F1 & F0
Which is peripheral and which is integral?- AF1 is peripheral
F0 is integral - BF0 is peripheral
F1 is integral
- AF1 is peripheral
14Name the function of ATP synthase
- AATP synthesis
- BATP hydrolysis
- CATP translocation
- DProton translocation
15If ATP is high, and the proton gradient is low, what will Complex V do instead?
- AHydrolyse ATP
- BSynthesise GTP
- CBreak itself down
16Protons flow through F1 to power the ATP synthesis in F0
- ATrue
- BFalse
17Name the subunits of F1
- AAlpha-Epsilon
- BA-E
- C1-5
18Which of the following subunit(s) are the largest in F1?
- AAlpha & Beta
- BGamma & Delta
- CBeta and Gamma
- DAlpha and Epsilon
19What is the role of the delta subunit in F1?
- AStabiliser for the F1 head
- BATP synthesis
- CConnets F1 too F0
20The epsilon subunit of F0 connects the gamma to the C subunit
- ATrue
- BFalse
21F0 contains how many subunits?
- A2- A & B
- B3- A, B & C
- C2- Alpha & Beta
22The F1 subunit is...
- A8nm long
- B18nm long
- C12nm long
- D90nm wide
- E19nm wide
- F9nm wide
23The Y subunit of F1 is made of 2 beta sheets that have been bent, but are bound at the base
- ATrue
- BFalse
24When the gamma subunit is in contact with a beta subunit, the ATP catalysis site is in it's _ formation
- AOpen
- BLoose
- CTight
25A subunit contains ADP + Pi- what conformation is it in?
- ALoose
- BTight
- COpen
26Whilst the subunit is replacing ATP with ADP + Pi, the gamma subunit remains in plance
- ATrue
- BFalse
27What order do the three conformations work in?
Assume you start with the Loose formation- ALoose-Tight-Open
- BLoose-Open-Tight
28State the characteristics of the C subunit of F0
- ASmall
- BLarge
- CHydrophobic
- DHydrophilic
- EIntegral
- FPeripheral
29How many monomers make up the C subunit?
- A8-12
- B8-10
- C4-10
30The C subunit contains 3 rings of monomer
- ATrue
- BFalse
31Why is there a variance across species of the number of monomers in the C subunit?
- AThe more complex an organism, the more efficient the subunit
- BThe more complex an organism, the less efficient the subunit
- CThe larger the organism, the more subunits are required
- DMammals have 8, birds have 12, all lesser animals and prokaryotes have 14 or more
32More subunits require more protons to move them, so more subunits is less efficient
- ATrue
- BFalse
33How is the A subunit described?
- AA proton half channel
- BA proton channel
- CA proton quarter channel
34Protons passing through the A subunit...
- AUse it to enter the C subunit and exit to the cytoplasm
- BUse it to exit into C subunit and enter from the cytoplasm
35Each subunit can hold 2 protons
- ATrue
- BFalse
36The momevent of the protons around the C subunit powers what?
- AThe movement of the gamma subunit
- BThe conformational changes of the beta subunits
- CThe attachment of the alpha subunits
- DThe rotation of the delta subunit
37The rotation of the F1 has been proved by attaching a microtubule filament to the F1 head and examining it under the microscope
- ATrue
- BFalse
38Only the gamma subunit of the F1 complex rotates
- ATrue
- BFalse
39How is the Alpha/Beta spheroid of F1 held in place?
- AB1 subunit
- BB2 subunit
- CEpsilon subunit
- DDelta subunit
40For every _degree rotation, 1 molecule of ATP is produced
- A90
- B120
- C180
- D360
41The mechanism of the gamma subunit causes an affinity change for ATP/ADP + Pi, driving the cycle
- ATrue
- BFalse
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- Description
- Structural Basis for Biological Function (Membrane Proteins) Quiz on Cyt C Oxidase & F1F0, created by gina_evans0312 on 18/12/2013.
- Author
- gina_evans0312
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- English
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