Quiz
Nonenzymatic Protein Function and Protein Analysis
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Questions in this quiz (15)
1At what pH can protein A best be obtained through electrophoresis? (Note: MM = molar mass)
- A2.5
- B3.5
- C4.5
- D5.5
2What is the function of sodium dodecyl sulfate (SDS) in SDS-PAGE?
- ASDS stabilizes the gel matrix, improving resolution during electrophoresis.
- BSDS solubilizes proteins to give them uniformly negative charges, so the separation is based purely on size.
- CSDS raises the pH of the gel, separating multiunit proteins into individual subunits.
- DSDS solubilizes proteins to give them uniformly positive charges, so separation is based purely on pH.
3Which of the following is NOT involved in cell migration?
- ADynein
- BFlagella
- CActin
- DCentrioles
4Which of the following proteins is mot likely to be found extracellularly?
- ATubulin
- BMyosin
- CCollagen
- DActin
5Hormones are found in the body in very low concentrations, but tend to have a strong effect. What type of receptor are hormones most likely to act on?
I. Ligand-gated ion channels
II. Enzyme-linked receptors
III. G protein-coupled receptors- AI only
- BIII only
- CII and III only
- DI, II, and III
6Which of the following is most likely to be found bound to a protein body?
- ASodium
- BPotassium
- CChloride
- DCalcium
7Which of the following characteristics is NOT attributed to antibodies?
- AAntibodies bind to more than one distinct antigen.
- BAntibodies label antigens for targeting by other immune cells.
- CAntibodies can cause agglutination by interaction with antigen.
- DAntibodies have two heavy chains and two light chains.
8Which ion channels are responsible for maintaining the resting membrane potential?
- AUngated channels
- BVoltage-gated channels
- CLigand-gated channels
- DNo ion channels are involved in maintenance of the resting membrane potential.
9Which of the following is NOT a component of all trimeric G proteins?
- AG⍺
- BGβ
- CGɣ
- DGi
10Which of the following methods would be best to separate large quantities of the following proteins? (Note: MM = molar mass)
- AIon-exchange chromatography
- BSize-exclusion chromatography
- CIsoelectric focusing
- DNative PAGE
11Which amino acids contribute most significantly to the pI of a protein?
I. Lysine
II. Glycine
III. Arginine- AI only
- BI and II only
- CI and III only
- DII and III only
12How does the gel for isoelectric focusing differ from the gel for traditional electrophoresis?
- AIsoelectric focusing uses a gel with much larger pore sizes to allow for complete migration.
- BIsoelectric focusing uses a gel with SDS added to encourage a uniform negative charge.
- CIsoelectric focusing uses a gel with a pH gradient that encourages a variable charge.
- DThe gel is unchanged in isoelectric focusing; the protein mixture is treated before loading.
13Which protein properties allow UV spectroscopy to be used as a method of determining concentration?
- AProteins have partially planar characteristics in peptide bonds.
- BGlobular proteins cause scattering of light.
- CProteins contain aromatic groups in certain amino acids.
- DAll organic macromolecules can be assessed with UV spectroscopy.
14A protein collected through affinity chromatography displays no activity even though it is found to have a high concentration using the Bradford protein assay. What best explains these findings?
- AThe Bradford reagent was prepared incorrectly.
- BThe active site is occupied by free ligand.
- CThe protein is bound to the column.
- DThe protein does not catalyze the reaction of interest.
15What property of protein-digesting enzymes allows for a sequence to be determined without fully degrading the protein?
- ASelectivity
- BSensitivity
- CTurnover
- DInhibition
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- Description
- MCAT Biochemistry Quiz on Nonenzymatic Protein Function and Protein Analysis, created by Sean Corcoran on 05/07/2016.
- Author
- Sean Corcoran
- Language
- English
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