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DISAGGREGATION OF EXPLANT

Keerthana Muruganandham English 5.0Average rating 5.0 out of 5 (2 ratings)

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  1. DISAGGREGATION OF EXPLANT

  2. MECHANICAL DISAGGREGATION

  3. ENZYMATIC DISAGGREGATION

  4. EDTA TREATMENT

  5. PRIMARY EXPLANT TECHNIQUES

  6. DISSECTION

  7. FINE CHOPPING

  8. WASH BY SETTLING

  9. TRANSFER TO BSS

  10. REMOVE BSS

  11. ADD THIN FILM OF MEDIUM

  12. INCUBATE FOR 2-3 WEEKS

  13. EXPLANT OUT GROWTH

  14. TISSUE SAMPLE

  15. CELL+DEBRIS

  16. MORE CELL + DERBIS

  17. VIABLE CELL+DERBIS

  18. DILUTE THE CELLS

  19. INCUBATE

  20. PRIMARY ADHERENT CELLS CULTURE

  21. SUB CULTURE

  22. CELL LINE

  23. CHOPPED

  24. WASHED

  25. COLD TRYPSINIZATION

  26. WARM TRYPSINIZATION

  27. TISSUE IN VIALS

  28. TISSUE PIECES+100ml TRYPSIN

  29. PIECES+TRYPSIN

  30. PLACE ON ICE FOR 1.6 hr

  31. DISAGGREGATE CELLS +TISSUE RESIDUES

  32. DISPERSED CELLS IN MEDIUM

  33. INCUBATE 35.6 DEG C

    20-30 MIN

  34. INCUBATE 35.6 DEG C

    48-72 HR

  35. DISPERSED CELLS

  36. PRIMARY CELL CULTURE

  37. COLLAGENASE

  38. CENTRIFUGE

  39. RESUSPEND

  40. WASH

  41. PRIMARY CELL CULTURE

  42. STEPS INVOLVED IN CELL CULTURE

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Description
Flowchart on DISAGGREGATION OF EXPLANT, created by Keerthana Muruganandham on 26/12/2017.
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Average rating 5.0 out of 5 (2 ratings)

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