Flashcards
Lecture Eight - Enzyme Kinetics: Michaelis -Menten Equation
Cards in this deck (18)
- 1Concepts to understandWhat is enzyme, enzyme terminology, and its function
The Six Classes of Enzymes
Michaelis—Menten Equation
Special cases of low and high (saturation) substrate concentrations
Lineweaver—Burk Equation and its use to determine v max and K - 2Enzymes ___ most of the biochemical reactions: Rate increase & Activity regulation Do not get ____ during the reaction Specificity (very ___) Systematic name: substrate name + type of reaction + 'ase'catalyzes
consumed '
specific - 3Enzyme ___ activation energy barrier to speed up reaction Enzyme is a catalyst that does NOT get ___ in reaction Enzyme does NOT change ____ constants Enzyme does NOT change the ___ energy difference between reactanct & productslowers
used
equilibrium
Gibbs - 4Enzyme terminology Substrate: The molecule on which an enzyme acts. Catalytic center or Active site: The specific site where the substrate ___ to an enzyme. Transition state: Substrate and enzyme form a ___. -->Complex energy is ___ compared to that of the substrate and products. Enzyme-substrate complex transforms into Enzyme-product complex to form final ____ _____: Molecules or ions that are essential for the catalytic activity, includes metals or small organic molecules, e.g., heme.binds
complex
higher
products
Cofactors
- 5The Six Classes of Enzymes Oxidoreductase: ___ -____ reactions Transferases: ___ transfer Hydrolases: ___ breakdown Lyases: ___ of groups by a mechanism other than hydrolysis and leaving behind double bonds Isomerases: Interconversion of ___ Ligases: Ligation or ___ of two substratesOxidation-reduction
Group
Hydrolytic
Removal
isomers
joining - 6Oxidoreductase: Oxidation-reduction reactions electrons are transferred Oxidation = __ electrons (lose H) Reduction = ___ electrons (gain H)lose
gain
- 7Transferases = group transfer
- 8Hydrolases: Hydrolytic breakdown (bonds are broken by using H2O)
- 9Lyases = REMOVAL of groups by mechanism OTHER than hydrolysis
- 10Isomerases = interconversion of isomers -->EX: D-alanine is used/produced by some bacteria
- 11Ligases = ligation or joining of two substrates
- 12—
- 13Michaelis - Menten Case [S] --> 0 Km ___ [S] so Km + [S] is about = Km Vo is about equal to __ / ___ First Order = V = k[S] = k[S]^1 rate equals ___>>
Vmax/Km[S]
k[S]![Michaelis - Menten Case [S] --> 0 Km ___ [S] so Km + [S] is about = Km Vo is about equal to __ / ___ First Order = V = k[S] = k[S]^1 r...](https://examtimeassets.s3.amazonaws.com/uploads/media/image/23935690/desktop_b0af95f5-f2ca-4472-844c-412082918836.png)
- 14Michaelis - Menten Case [S] --> infinity , Saturation km ___ [S] so Km + [S] is about equal to [S} Vo = Vmax = K2[E]total Zero Order = V = k [S]^0 Rate = k Vo = ___<<
k
Vmax![Michaelis - Menten Case [S] --> infinity , Saturation km ___ [S] so Km + [S] is about equal to [S} Vo = Vmax = K2[E]total Zero Order =...](https://examtimeassets.s3.amazonaws.com/uploads/media/image/23935783/desktop_bd90c7c3-57c1-4bb2-b2d2-932f309687fd.png)
- 15Michaelis - Menten Case [S] ---> infinity Vmax = kn[E]total = kcat[E]total kcat = ___ constantcatalytic
- 16Michaelis - Menten Case vo =Vmax/2 where rate = 1/2 [S] = ___ High Km = ___ affinitykm
low![Michaelis - Menten Case vo =Vmax/2 where rate = 1/2 [S] = ___ High Km = ___ affinity](https://examtimeassets.s3.amazonaws.com/uploads/media/image/23935812/desktop_42d774ae-9191-441c-b7c4-b02fc1ab7c85.png)
- 17—
- 18Double reciprocal Lineweaver-Burk Plot = Permitted experimental ____ of Km and Vmax without the use of a computerestimation
