Flashcards
Qualitative and Quantative Analysis in Chromatography
Cards in this deck (12)
- 1Qualitative AnalysisAny method of identifying the chemical components in a sample.
- 2Quantitative AnalysisWorking out how much of a given chemical component there is in a sample.
- 3ChromatographyThe separation of the components of a mixture dissolved in a liquid or gas (the mobile phase) carrying it through a structure holding the stationary phase.
- 4AdsorbentOften used to describe the stationary phase in chromatography because substances become adsorbed to it during separation.
- 5AdsorptionWhen a substance (e.g. a gas, liquid or solute) binds to or attaches to another, usually solid.
- 6PolarA polar (hydrophilic) substance will dissolve in or mix with water.
- 7ElutionTo wash out. In column chromatography this means 'washing out' a substance that has become adsorbed to the column (stationary phase).
- 8EluentThe solvent (mobile phase) used to wash substances out of a column.
- 9EluateThe mobile phase, containing dissolved substances, as it emerges from a column.
- 10Gas Chromatography (GC)A separation technique using an inert carrier gas as the mobile phase and a thin layer of liquid or polymer on an inert solid support as the stationary phase.
- 11High Performance Liquid Chromatography (HPLC)A type of column chromatography that uses very small particles and high pressures to achieve better separation. It can be used for analysis or, on a larger scale, for purification.
- 12Gel ElectrophoresisThe use of an electric field for separation of compounds based on differences in their charge and size. Usually for DNA.
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- A level Applied Science Flashcards on Qualitative and Quantative Analysis in Chromatography, created by Rebecca Hedworth on 15/04/2018.
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- Rebecca Hedworth
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