Flashcards
Conjugation
Cards in this deck (16)
- 1Method of TransferMovement of DNA by direct cell to cell contact
- 2Recipient of DNAExconjugant
- 3Form of DNA TransferredUsually a plasmid, or as conjugated transposons (these must integrate into chromosomes)
- 4How do plasmids pick up new genes?Transposition (genes capable of independent replication) or excision from/insertion into the chromosome
- 5Stage 1 of ConjugationSurface Adhesions (Gram +) or Conjugative Pili (G-) mediate donor-cell interactions
- 6Stage 2 of ConjugationMating Pair Junction is formed
- 7Stage 3 of ConjugationDNA (usually ss) is transferred
- 8Stage 4 of ConjugationThe recipient synthesizes a complimentary strand to the ss DNA gained, as does the donor
- 9Transfer Apparatus- RelaxosomeContains OriT & Relaxase
- 10Transfer Apparatus - Role of RelaxaseCleaves DNA at OriT & binds to 5' of transferring strand
- 11Transfer Apparatus- Type IV Secretion SystemEncoded by mpf genes, it recognises Relaxase, not DNA
- 12Transfer Apparatus- Connective Coupling ProteinConnects Relaxosome & T4SS
- 13Key Conjugative Plasmid Genes- Stability GenesControl segregation
- 14Key Conjugative Plasmid Genes- Replication genesCo-ordinate replication with host genome (so it replicates once per cell cycle)
- 15Key Conjugative Plasmid Genes- Propogation genesEnsure correct transfer between cells
- 16Key Conjugative Plasmid Genes- Adaptation geneThe genes that confer the advantage
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- Medical Microbiology (Gene Transfer) Flashcards on Conjugation, created by gina_evans0312 on 08/02/2014.
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