Flashcards
Microscopes
Cards in this deck (12)
- 1ResolutionAbility to differentiate between 2 close objects
- 2MagnificationHow big an image is compared to the object
- 3Equation for magnification
- 4Decribe optical microscopeLight focused by lens
Light passed through specimen
More light absorbed = darker
Mag = Eyepiece x Objective lens - 5Advantages of optical microscopeCheap
Easy to use
True colour (may req staining) - 6Limits of optical microscopeLow resolution (0.2um)
Low magnification (1500x) - 7Decribe SEMsDirects beam of e- across specimen
Image based on e- reflected
( Knocks of e- from spec
Gather in cathode ray tube
form image on comp ) ADDITIONAL - 8Advantages of SEMsHigh resolution (200,000x)
Surface structure 3D
High magnification (2nm)
Thick spec - 9Limits of SEMsLower resolution than TEM
Dead spec
Expensive
Training
B&W / False colour - 10Decribe Transmission Electron MicroscopeUse electomagnets to focus beam on e- transmitted through spec
image based on e- absorbed
Denser spec = more e- absorp = darker - 11Advantages of TEMsHigh resolution (2nm)
images on internal structures
High magnification (500,000x) - 12Limits of TEMsThin spec
Dead spec
Expensive
Training
B&W / False colour
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- A-Level Biology (Cell Structure) Flashcards on Microscopes, created by Nazmin Shafiq on 26/11/2016.
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- Nazmin Shafiq
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