DNA Concept Map

DNA

cytoplasm

nucleus

double-stranded

double-stranded

+/- stranded

complex

enveloped

linear

Inverted terminal repeat sequences at ends fold over 3’ end used to initiate DNA replication

covalently linking ends combined with inverted terminal repeats ends replication

viral DdDp & DdRp & poly A pol

Poxviridae I

Genome: 130-280 kb

Capsid (d): 17-200x300-450 nm

icosahedral capsid

nonenveloped

linear genome

passes directly though NPC due to size

reproduce in highly replicative cells

Inverted terminal repeat sequences at ends fold over 3’ end used to initiate DNA replication

•Inverted terminal repeat sequences at ends fold over 3’ end used to initiate when get to the other end, nick unfold and copy to replicate virus ends

Host RNA & DNA pol

Parvoviridae II

Genome: 5 kb

Capsid (d): 18-26 nm

enveloped

cccDNA

Capsid enter NPC but only dsDNA enter nuclues

Protein primer binds to 5’ end Serine, threonine or Tyrosine provide OH’ group on which DNA polymerase can add nucleotides to initiate DNA replication

Viral Proteins:

envelope proteins

Packaged:

viral-pol (RT)

linear genome

capsid binds NPC and genome is threaded through

Origin of replication opened by helicase, primase adds RNA primers to provide 3’ OH to initiate DNA replicaiton

Make your own DNA pol but still replicate in the nucleus and use the host cell replication machinery

Viral Proteins:

MHC Downregulation

protease

Hepadnaviridae I

Genome: 3.2 kb

Capsid (d): 42 nm

Herpesviridae I

Genome: 120-200 kb

Capsid (d): 150-200 nm

nonenveloped

viral enzymes to enter S-phase

icosahedral capsid

linear genome

capsid binds NPC and genome is threaded through

assembles in nucleus

Make your own DNA pol but still replicate in the nucleus and use the host cell replication machinery

viral protease

Protein primer binds to 5’ end Serine, threonine or Tyrosine provide OH’ group on which DNA polymerase can add nucleotides to initiate DNA replication

•Protein primer binds to 5’ end Serine, Threonine or Tyrosine provide OH’ group on which DNA polymerase can add nucleotides to replicate virus ends

Adenoviridae I

Genome: 36-38 jb

Capsid (d): 18-26 nm

circular genome

capside shed as enters the nucleus

Origin of replication opened by helicase, primase adds RNA primers to provide 3’ OH to initiate DNA replication

Entry:

glycosaminoglycans

Exit:

lysis, budding

Entry:

endocytosis

Exit:

Lysis

Entry:

cell-receptor endocytosis

Exit:

budding

Entry

glycoproteins

cell receptor endocytosis

Exit:

budding

(reverse budding)

Entry:

co-receptor mediated endocytosis

Exit:

lysis

Entry:

cell receptor endocytosis

Exit:

lysis

Polyomaviridae I

Genome: 5 kb

Capsid (d): 40-50 nm

assembles in nucleus

Viral Proteins:

tumor antigens

capsid proteins

agnoprotein

Papilomaviridae I

Genome: 8 kb

Capsid (d): 40-55 nm

Entry:

cell receptor endocytosis

Exit:

lysis