Flashcards
Corynebacterium
Cards in this deck (26)
- 1What is the microscopic appearance of corynebacterium?pleomorphic- mostly rods/cocci forming palisades.
Non-motile
Gramm + - 2What is the incubation/ plating method?Mesophiles with 2 day incubation,facultative anaerobes.
Plating on blood agar-enriched - 3What is important component of their cell wall?high concentrations of lipids.
Meso-diaminopimelic acid.
Arabinogalactan.
Mycolic acids. - 4Results of catalase and oxidase test?Cat +
Ox - - 5What is the urease test and what are its results?Tests the ability of the bacterium to secrete urease enzyme, which hydrolyses urea to ammonia and carbon dioxide.
Ammonia released causes mucosal inflammation.
All corynebacterium are + except C. Bovis. - 6Is it possible to treat Corynebacterium with antibiotics (β lactams)?Yes but only in early infection
Not in sheeps! - 7Describe colony of corynebacterium:Small
C. renale= yellow
C. pilosum= yellow
C. cystitidis= white
C. Pseudotuberculosis= white with β-hemolysis after 48-72h incubation. - 8Are corynebacteria usually commensal?Yes in mucous membranes and can cause pyogenic infections
- 9What are usual samples used for testing?tissue
exudate
pus
mid-stream urine - 10What tests do we usually do?PCR, ELISA
CAMP for C. Pseudotuberculosis and Rhodococcus Equi - 11What are the 2 biotypes of C. Pseudotuberculosis?1) nitrate reducing
2) non-nitrate reducing - 12Which corynebacterium has affinity for lymphatic system?C. Pseudotuberculosis
- 13Which animals are affected by the non-nitrate reducing biotype? How?Sheep and goat.
It causes lymphadenitis- abcess formation in lymph nodes or internal organs. - 14Which animals are affected by the nitrate reducing biotype? How does the infection occur?Cows and horses.
The infection occurs by skin wounds, insects or by contact. - 15The non-nitrate biotype is spread by: Is it treatable?pus, nasal and oral secretions.
It is not treatable - 16Ulcerative lymphangitis usually affects (animals:)_____and one major characteristic is_______It affects cows and horses.
Characteristic is chronic abscesses with greenish pus and blood tinged. - 17Which strain of corynebacterium affects the lower urinary tract of cattle?C. Renale
- 18What is the disease that when it's too serious the kidney must be removed and the patient will need dialysis? Which bacteria cause this disease?Pyelonephritis- inflammation of kidney tissue- calyces and renal pelvis.
It is caused by C. Renale. - 19Unvaccinated individuals and children are more likely to be ill after infection by___?___C. Diphtheria
- 20How many subspecies does C. Renale have?3
C. Renale
C. Pilosum
C. Cystitidis - 21Effects of C. Renale in different species:- cystitis & pyelonephritis in BO
- ulcerative balanoposthitis in OV+CAP (inflammation of glans penis) - 22What toxin by which strain causes cell death?DT toxin by C. Diphtheria
- 23Where can we see hemolysis?C. pseudotuberculosis
- 24Where can we find urease?All pathogenic strains except C. bovis
- 25How does DT toxin work?AB toxin= 2 components: one for activation, one for binding.
It is encoded by a bacteriophage and secreted when Fe levels are low.
*It enters the cytoplasm of cell and inhibits protein synthesis- DNA/RNA decreased- energy metabolism also affected=> CELL DEATH
If the toxins reaches the organs via bloodstream it can cause paralysis and congestive heart failure. - 26Which strain produces exotoxins and phospholipase D?C. Pseudotuberculosis
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- Microbiology Flashcards on Corynebacterium, created by Sotia P. on 14/04/2019.
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- Sotia P.
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