Flashcards
Biochemistry exam A
Cards in this deck (8)
- 1Why are X-rays used for crystallography?The wavelength of X-rays is near that of the
bond length of most atomic bonds (think of ruler) - 2What are some measures of X-ray crystal structure quality?R factor
Electron density quality
Resolution - 3What does Braggs Law describe?Bragg’s Law Describes how Incident Xrays
and Diffracting Waves are Related - 4What are the variables of Braggs law?n is the number of wavelength between two rows of atoms
Lambda is the wavelenght
D is the spacing between the lattice planes
Theta is the angle between in the incident ray and the scattering planes - 5Name three (3) benefits and/or challenges with the use of peptidomimetics as therapeutic molecules.Constrained molecules minimise entropy cost
More stable than “classical” peptides
Relatively easy to synthesise - 6Outline the steps involved in the interaction, binding and release of peptides and proteins by Hsp70 during the protein folding process.• Hsp40-mediated delivery of
peptide to ATP-bound Hsp70
• Hydrolysis of ATP accelerated
by Hsp40 resulting in helical lid
closure & tight binding of
peptide
• NEF catalyses dissociation of
ADP
• Binding of ATP induces lid
opening & release of peptide
• Released peptide either folds to
native state (N) or is transferred
to downstream chaperones or
rebinds to Hsp70 - 7Describe the structural features of the intermediately folded, molten globule state of a protein.– compact globule, yet expanded over a native radius (~10-30%)
– native-like secondary structure, can be measured experimentally
– limited tertiary structure, highly dynamic
– exposed hydrophobic core - 8Describe one alternative target to HIV protease for inhibiting HIV.Reverse Transcriptase
Intergrase