Electron Transport Chain

alexlpeart
Flashcards by alexlpeart, updated more than 1 year ago
alexlpeart
Created by alexlpeart about 8 years ago
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Exercise Metabolism Flashcards on Electron Transport Chain, created by alexlpeart on 04/29/2013.

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Question Answer
Give a problem with using NADH and FADH2 in the ETC. What system do we used as a solution to this problem. NADH and FADH2 cant cross the mitochondrial membrain. We have to use the Glycerophosphate shuttle system to get electrons from NADH to the inside of the cell.
Describe the mechanisms in the glycerophosphate shuttle.
Describe an alternative method of getting NADH into the mitochondria instead of using the glycerophosphate shuttle.
What two high energy molecules do we add to the ETC? NADH and FADH2
Where does Glycolysis, TCA cycle and the ETC take place? Cytoplasm, Matrix, Inner Membrain
Briefly describe the electron transport chain. Electrons are carried by reduced co-enzymes are carried down a chain of proteins located in the mitochondrial matrix. This movement causes a proton gradient to form as hydrogen ions are pulled across the matrix. This gradient is then used to create ATP molecules from ADP
Which ETC complex does the electron leave the chain? Cytochrome C Oxidase (complex IV)
Why does FADH2 pull less protons across the matrix than NAHD. The electrons from NADH enter the ETC through NADH dehydrogenase (complex I) while FADH2 enters the ETC at succinate dehydrogenase (complex II). Succinate dehydrogenase also doesn't straddle the membrain so cant draw protons across the matrix like NAHD dehydrogenase can.
How many protons does NADH and FADH2 pull across the membrain? How many protons do you need to make one ATP molecule? So how many ATP can you make from each NADH and FADH2? NADH pulls 10 protons across while FADH2 pulls 6. We need 4 protons to make one ATP so NADH creates 2.5 ATP while FADH2 creates 1.5 ATP.
Describe the path an electron would take if it were coming from an NADH molecule. Use the correct names for each complex and try to include transporter proteins which travel within the membrain. Electron enters at NAHD dehydrogenase , passes to Succinate dehydrogenase where it is passes to a transport protein called the quinone pool (Q). This transfers the electron to cytochrome b-c1, then via a transport protein to cytochrome C oxidase where it leaves the electron transport chain.
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