Flashcards
RAS Activation
Flashcards do conjunto (38)
- 1While inactive, tyrosine kinase exists asA monomer
- 2The binding of the ligand causes the ....?... of tyrosine kinaseDimerisation
- 3The structural change of tyrosine kinase causesPartial activation
- 4What allows tyrosin kinase to become fully active?The phosphorylation of the activation lip
- 5When the protein is fully activation, which protein is phosphorylated?Tyrosine kinase
- 6This phosphorylation provides a docking site for which protein?GRB2
- 7What does the activation of RAS require?A complex of protein linked to tyrosine kinase
- 8Which part of GRB2 binds to GEF?SH3
- 9The SH2 subunit of GRB2 binds where?To the phosphorylated tyrosine kinase
- 10Why doesn't GRB2 bind to GEF all the time?Because only binding to phoshorylated T.K exposes the SH3 domain
- 11Adaptor ProteinAllows 2 unlike proteins to bind
- 12What aa-rich domain does the SH3 domain of GRB2 bind to?The proline rich region of GEF
- 13Where is inactive RAS found in a cell?In the membrane bound to GDP
- 14What does GEF stand for?Guanine nucleotide exchange factor
- 15Why does GEF binding activate RAS?It replaces GDP with GTP, activation it
- 16What is the main function of active RAS?Downstream cascade causing cell division
- 17How is RAS deactivated?GAP- GTPase activating protein causes GTP hydrolysis
- 18How long is RAS active for (in general)One minute
- 19How does GEF activate RAS?Inserts an alpha helix to open the switches, so GDP is replaced by GTP
- 20Name the two aa that hold the RAS switch closedGly60 & Thr 35
- 21What is the first protein RAS activates?RAF
- 2214-3-3 proteinBinds to RAF and holds it in a folded state
- 23Phosphorylation of what aa holds RAF inactive?Serine
- 24The binding of RAS to RAF causes what to be displaced?14-3-3 protein
- 25RAS binding causes what to be phosphorylated?Kinase domain
- 26RAS binding causes what to be dephosphorylated?The N terminus
- 27Once RAF has been unfolded, it phosphorylatesMEK
- 28MAPK is phosphorylated byMEK
- 29MEK is unusual becauseIt can phosphorylate Ser, Thr & Tyr
- 30Function of MPKPhosphorylation of nuclear transcription factors
- 31How is MAPK activated?Phosphorylation of the activation lip
- 32Name the P protein activated by MAPKp90-rsk
- 33p90rsk activatesPIPG
- 34MEK stands for...MAP Kinase Erk Kinase
- 35MEK stands forMAPK Erk Kianse
- 36How does MEK phosphorylated MAPK?Tyrosine residue on activation lip, then the newly exposed Threonine residue
- 37p90rsk is sensitive to...Insulin
- 38PP1G stands forProtein phosphatase-1-G
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- Year 2 FlashCards sobre RAS Activation, criado por gina_evans0312 em 04-11-2013.
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- gina_evans0312
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