Flashcards
BMS04-1038- Control of gene expression 1
Flashcards do conjunto (14)
- 1When is gene expression regulated?1. Cell differentiation/development
2. In response to environment (food/hormones) - 2What is the major point of gene expression regulation?Transcription
- 3What are transacting molecules? What do transacting molecules bind to?Molecules (usually proteins) that are synthesised by a gene other than the targetted one
Bind to Cis acting elements by diffusing through cell - 4What type of molecule is General Transcription Factors that initiate transcription?Transacting molecule
- 5What are regulatory sequences? Where are they located? Why are they considered "cis"? What is an example?1. Short DNA sequences in noncoding regions that control transcription initiation
2. Cis because they act on genes on the same DNA molecule
3. TATA Box - 6What is the TATA Box, where is it, and what does it do?Short consensus sequence from 34-26 UPSTREAM the start codon (can be in promoter region)
Positions RNA Polymerase II for transcription initiation - 7Where is the promoter found in relation to the coding region? What occurs at this region?The promoter is found in the 5' upstream section of the coding region
The transcription initiation complex is assembled - 8What do general transcription factors do?Bind DNA to form the preinitiation complex+recruit RNA Polymerase II at the promoter region
- 9What do sequence specific DNA binding TFs do? Where do they bind?enable/prevent specific promoters
Upstream promoter sequence - 10What are two ways hormones can influence gene expression?1. Bind to receptors on cell membrane, starting a signalling cascade
2. Bind to receptor, which translocates to nucleus to stimulate transcription initiation - 11How can gene expression be regulated during the polyadenylation of RNA?UIA protein binds to own mRNA to inhibit polyadenylation (negative feedback loop)
- 12How does differential/alternative splicing of RNA allow for gene regulation?Different EXONS on same gene selectively removed to create different DNA sequences
- 13How does fibronectin differ in the liver and fibroblasts?Fibroblasts make ADHESIVE fibronectin that sticks to ECM
Liver makes fibronectin w adhesive properties spliced out, so can circulate in body - 14Why does gene expression need to be controlled?Wasteful to make unneeded proteins
Informações
- Descrição
- The reasons for regulating gene expression. The different levels of gene regulation in eukaryotes. Regulation of RNA expression during transcription initiation. cis sequences and trans acting factors. Promoter, TATA box, transcription factors. How general transcription factors and RNA Pol II bring about constitutive gene expression. Regulation by hormones. Examples of regulation of RNA expression during poly-adenylation and splicing. What happens when gene regulation is dys-regulated.
- Criado por
- Evian Chai
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- 24
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