Microscope

Page 1

Parts of a microscope-

Eyepiece- for the viewer to look to see the specimen

Objective lens- collects the light coming through the sample magnifies it

Mirror- allows to t the light and illuminate the specimen

Condensor lens - used to focus light on the specimen

Revolving nose piece- holds the objective lens in place

Iris diaphragm- controls the amount of light that reaches the aperture

Stage- the platform where you place the slide

Slide- a flat piece of glass used to hold the specimen in place

Adjustment knobs ( coarse and fine )- for focusing and fine-tuning

Coverslip- to keep the specimen flat and avoid evaporation

Page 2

Meanings -

Cytoplasm- It is a substance that fills the cell. It is found inside the cell membrane surrounding the cell nucleus.

Cell wall- It protects the cell membrane.

Page 3

Slide Preparation-

Place the specimen on the slide

Add a drop of staining specimen to the slide. ex- safranin, methylene blue

Cover the specimen with a coverslip

Important- safranin is used for plant cells and methylene blue for animal cells

Page 4

Steps to adjust the microscope-

Adjust illuminator mirror to get maximum light

Rotate low power objective into place

Place the slide onto the stage - centre the specimen over the hole in the stage. Secure the slide with stage clips

Look through the eyepiece and turn the coarse adjustment knob until the specimen comes clearly into view. Adjust slide if necessary. Use fine adjustment knob for fine-tuning

Draw what you see in the field of view

Rotate high power objective into place and repeat steps 4 and 5 using fine adjustment knobs only