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MO20 Quality Control III

Mer Scott 14 preguntas

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Preguntas del test (14)

  1. 1In Normal phase HPLC, the stationary phase is [blank_start]polar/hydrophilic[blank_end] and elution composition is a mixture of [blank_start]non-polar and more polar organic[blank_end] solvents. Therefore the elution order is most [blank_start]hydrophobic[blank_end] first then more [blank_start]hydrophilic[blank_end]. The interactions involved are [blank_start]hydrogen bonding[blank_end].

    In Reverse phase HPLC, the stationary phase is [blank_start]non-polar/hydrophobic[blank_end] and elution composition is a mixture of [blank_start]organic and aqueous[blank_end] solvents. Therefore the elution order is [blank_start]most hydrophilic[blank_end] first then [blank_start]more hydrophobic[blank_end]. The interactions involved [blank_start]are hydrophobic[blank_end].
    1. Apolar/hydrophilic
    2. Bnon-polar/hydrophobic
    3. Cnon-polar and more polar organic
    4. Dhydrophobic
    5. Ehydrophilic
    6. Fhydrogen bonding
    7. Gorganic and aqueous
    8. Hmost hydrophilic
    9. Imore hydrophobic
    10. Jare hydrophobic
  2. 2Choose the incorrect statement about Normal Phase HPLC/TLC.
    1. ASilica is used as the stationary phase
    2. BSilica forms hydrogen bonds with functional groups on drug molecules (eg. OH, NH etc.)
    3. CSilica has low affinity for polar analytes
  3. 3What could you do to increase the separation and resolution of peaks of elution in Normal Phase HPLC?
    1. AIncrease non-polar solvent
    2. BDecrease non-polar solvent
  4. 4In Reverse Phase HPLC, resolution can be improved by changing the mobile phase composition. You:
    1. Aincrease the polar solvent %
    2. Bincrease the aqueous solvent %
  5. 5[blank_start]Isocratic[blank_end] elution is where the composition of the mobile phase stays constant over time.
    [blank_start]Gradient[blank_end] elution is where the composition of the mobile phase changes over time.
    1. AIsocratic
    2. BGradient
  6. 6When looking at HPLC results, retention time can provide information about [blank_start]identity and purity[blank_end] if compared to a [blank_start]standard[blank_end]. The area under the peak or peak height can be used to quantify [blank_start]amount[blank_end] of drug (and also measure [blank_start]purity[blank_end]) by using a [blank_start]standard or the Beer-Lambert Law.[blank_end]
    1. Aidentity and purity
    2. Bstandard
    3. Camount
    4. Dpurity
    5. Estandard or the Beer-Lambert Law.
  7. 7Which of these is not a method of sample preparation before comparison to a standard?
    1. AProtein Precipitation and centrifugation
    2. BLiquid-liquid Extraction (LLE)
    3. CSolid Phase Extraction (SPE)
    4. DGas-Liquid extraction (GLE)
  8. 8The External Standard Method: Solutions of [blank_start]known[blank_end] concentration are prepared that contain standards of the [blank_start]same[blank_end] drug to be analysed. The drug being analysed can be identified by its [blank_start]tR[blank_end] and its concentration can be determined from the [blank_start]peak area[blank_end] relative to that of the standard.
    - When using the single standard external standard method we use the equation C [blank_start]sample[blank_end] = ([blank_start]A sample/A standard[blank_end]) * C [blank_start]standard[blank_end]
    - When using the calibration curve external standard method, we use the equation [blank_start]y = bx + a[blank_end]
    1. Aknown
    2. Bsame
    3. Cretention time
    4. Dpeak area
    5. Esample
    6. Fstandard
    7. GA sample/A standard
    8. HA standard/A sample
    9. Iy = bx + a
  9. 9The Beer-Lambert Law only holds true if the calibration curve is linear.
    1. AVerdadero
    2. BFalso
  10. 10Choose the incorrect statement about the internal standard method.
    1. AIt involves a compound that is different to the analyte of interest
    2. BStandards contain the different concentrations of internal standard (IS) and the same concentration of analyte [X]
    3. CIt is used only in assays involving separation

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Descripción
(Molecules) PHCY211 Test sobre MO20 Quality Control III, creado por Mer Scott el 14/04/2018.
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