Flashcards
Microbiology Practical I
Cards in this deck (14)
- 1Different Types of MicroscopyBrightfield
Phase-contrast
Darkfield - 2What objective lens do you use first?10x
40x
then 100x - 3What is not used for stained smears?NO COVER SLIPS
- 4How do you dry a simple stain?let slide air dry and then heat fix the smear by running thru the fire.
- 5What do you flood the simple stain?Crystal violet then wash the slide with distilled water
- 6What do you use to blot a smear slide?bibulous paper pad
- 7What are the 3 types of condensers for 3 types of light microscopy?-brightfield (A on condenser)
-darkfield (DF on condenser)
-phase-contrast (condenser settings: PH 1 for 10X, PH3 for 40X, PH4 for 100X) - 8What do you always start with when you look at a specimen on the microscope?You always start with brightfield microscopy
- 9What is the condenser?gathers all available light from the lamp and directs it up
to the stage - 10When viewing largest objects, like worms or insects, you can movethe condenser down to improve light density hitting the specimen, but not for microorganisms.
- 11Iris diaphragmYour light amount coming up through
the condenser - 12How much do you turn the brightness control knob?ALL the way up, and then back off 1/4 of a turn. This is
where the control knob will stay: do not touch it again. - 13Rotate revolving nosepiece to 10x then?use coarse adjustment knob to bring stage all the way up.
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