Flashcards
Microbiology Lab Notes
Cards in this deck (74)
- 1Chemically defined mediumthe composition and amount of each ingredient is known.
- 2Chemicaly undefined mediumThe composition and amount of each ingredient is not known.
- 3Enriched MediumAdditional nutrient added for encouraging the growth of fastidious MO's
- 4Differental MediumDifferentales between Mo's based on ability to conduct a metabolic process
- 5Selective Medium contains ingredientsthat select for a particular type or group of Mo's
- 6Maintence Mediumallows for maintaining organisms for a long period of time
- 7Agarcomposed of polysaccharide isolated from red algae
- 8Agar-melts at 100*c and solidifies at 45*c
-most Mo's are unable to utilize agar as a carbon and energy source
-Utilized to convert medium to solid medium - 9AutoclaveA fancy, expensive pres
- 10Aseptic Techniquethe process of handling sterile material in a manner that prevents contamination
- 11ContaminationA situation where unwanted Mo's end up in sterile materials or cultures due to the lack of proper aseptic technique
- 12Why do we flame a loopto prevent contamination
- 13Why do we write on bottom of the plateThats where the media is
- 14Why do we incubate media upside down?To prevent contamination
To prevent condensation - 15Purpose of a streak plateIsolate pure culture
Determine if a culture is pure - 16How do you know if you have a pure culture on a plateSame characteristics (color, size)
- 17Non pathogenic Mo'sdosent cause disease
- 18Pathogenic Mo'scause disease
- 19Opprotunistic Mo'sneeds help getting in
- 20Udipodisfound everywhere
- 21Hostwho has disease
- 22Reservoirwhere does the pathogen survive grow reproduce and spread from
- 23Aerotoleranceindicates the ability or inability of Mo to grow in the presence of oxygen
- 24Obligate aerobesorganisms that has to have oxygen to grow
(grows faster in aerobic in conditions) - 25Faculative Anaerobesgrows in the presence with oxygen or without
( grows faster in aerobic conditions) - 26Aerotolerantgrows in the presence of oxygen or without
( grows at the same rate in conditions) - 27Obligate Anaerobecannot grow with oxygen
- 28Microaerophilesreduced rate of oxygen
- 29Capnophilesneeds carbon dioxide
- 30Fluid Thioglycollate Mediumcontains yeast extract glucose casein
- 31Agarslows down the diffusion of oxygen into a medium
- 32Yeast Extractprovides vitiamin and minerals for bacterial growth
- 33Glucose and Caseinserve as carbon and energy sources
- 34Sodium Thioglycollate and L cystinereduces free oxygen to water making oxygen available
- 35Methelyene Blue Indicatoris blue when oxidized (presence of oxygen) and colorless when reduced ( absence of oxygen)
color- aerobic
colorless- anaerobic - 36Cardinal TemperaturesMinimum, optimum, maximum
- 37Psychrophilesgrows 0 to 20 *c
- 38Mesophilesgrow 20 to 40 *c
- 39Thermophiles (obligate and facultative)grow 45 to 70 *c
- 40Extreme Thermophilesgrow 70 to 100 *c
- 41Hyperthermophilesgrows 100 to 150* c
- 42pHmeasure of hydrogen ion concentration
Increase H solution becomes more acidic and ph goes down
Increase H solution becomes more basic and ph goes up
Mo's pH ranges 0.5 to 12.0 - 43Acidophilesbetween 0.5 to 6.0
- 44Neutrophilesbetween 6.0 to 7.5
- 45Alkaliphilesbetween 7.5 to 12
- 46Osmotic/ Osmosiswater active ( solute present)
movement of a solvent from high concentration to low concentration until equlibrium is reached - 47Lysisrupture of cells
- 48Plasmolysisshrinkage of cells
- 49Hypertonicsolutions
- 50Halophilessalt loving Mo's
.5% to 3% - 51Moderate halophilesgrows at a concentration greater than 3% Nacl
- 52Extreme HalophilesExtreme Halophiles
- 53Turbiditymeasure of cloudness in water or growth in a halophile
- 54substage condensercondenses the beam of light onto the specimen
- 55Iris diaphramcontrols light intensity of beam on a specimen
- 56Stageflat surface on which microscope slide rests
- 57Mechanical Stageallows movement of slide in all directions allows minute movement of slide
- 58Objective Lensesmagnifies specimen and produces the real image
(4x) scanning 10x (low power) 40x ( high dry) 100x (oil immersion) - 59Revolving nose pieceenables moving different objectives lenses into position
- 60Ocular Lensestwo ocular lenses ( binocular)
- 61Turret and Set screwallows loosening and rotation of oculars and locking of turnet in position
- 62Fine Focus and Course Knobsfocus adjustment
Large Knob- coarse focus
Small Knob- fine focus - 63Total Magnificationocular power times objective power
4x=40
10x=100
40x=400
100x=1000 - 64Resolution or Resolving Powerthe ability of the objective lenses to distinguish two points as clear and specific distance apart
RP= .5 timess lambda / N.A
.5 is constant - 65Resolving PowerRP= .5 timess lambda / N.A
.5 is constant - 66How to improve resolving ituse shorter wavelength of light to improve resolution of microscope
use immersion oil with 100x to change index refraction - 67Name of microscopecompound microscope or compound bright microscope
- 68Problems that can arise that affects ones use of microscopefield of view is dark
-check to see that light is on - 69Basic Stainstain the bacterial cells ( have negative charge) but does not stain the slide ( has positive charge)
Positevly charged dyes - 70Acidic StainStain background ( has positive charge) but do not stain the bacterial cells (have negative charge)
- 71Simple Stainuses only one dye
Procedure is used to determine size of cells morphology of cells and arrangement of cells - 72Differntal Stain 2 or more dyespurple in color ( crystal violet)
Iodine ( Grams Iodine)
Decolorization ( 95% ethanol)
Saphrine ( Secondary) - 73Differental Staining4 stages
primary, mordant, decolorization, counterstain - 74DecolorizationSmear thickness
too thick of a smear can lead to erroneous results.
Too thin of a smear can result in immediate decolorization of Gram positive bacteria
Information
- Description
- Microbiology Flashcards on Microbiology Lab Notes , created by Jayla Williams on 24/09/2019.
- Created by
- Jayla Williams · Sep 2019
- Updated
- Sep 25, 2019
- Views
- 0
- Rating