Adenylate Cyclase & Why cAMP

Question	Answer
A monomer/dimer/heterodimer?	Dimer
How many membrane spanning loops in each monomer?	6
Where is the catalytic site?	Inside the cell
What activates adenylate cyclase?	The alpha subunit of Gs protein
What effect does the binding of the alpha subunit have?	The two halves of the catalytic site combine and activate
What does adenylate cyclase produce?	3,5-cAMP
Why cAMP? A variety of hormones...	can activate the same pathway in different tissues
Why cAMP? A hormone can activate...	Different pathways in different tissues
Why cAMP? The same hormone doesn't have to...	Activate the same pathway, even if the pathway's only regulated by one mechanism
What is cAMP (as part of the cascade)?	A secondary messager
What effect does the cholera toxin have on Adenylate Cyclase?	Binds ADP-Ribose to the alpha-s subunit, which prevents it rebinding to the g-protein
What effect does the pertussis toxin have on g-protein alpha subunit?	Binds ADP-Ribose to the alpha-i subunit, which prevents it separating from the g-protein

Resource summary

	Created by gina_evans0312 over 10 years ago