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Description
Flashcards by harrisoncr, updated more than 1 year ago
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Created by harrisoncr
over 8 years ago
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| Question | Answer |
| Describe the function of the following parts of the microscope: -ocular lens | Lenses through which the object on the stage is viewed; may be monocular or binocular; usually provides additional 5x or 10x magnification |
| Describe the function of the following parts of the microscope: -objective lens | Lenses of different power to magnify the object on the stage; usually 10x, 40x, and 100x power |
| Describe the function of the following parts of the microscope: -iris diaphragm | Adjusts the amount of light entering the condenser |
| Describe the function of the following parts of the microscope: -condenser | Collects and concentrates light upward through the object on stage |
| List three things they must do in order to put away their microscopes correctly. | 1) Remove slide from stage 2) Wipe away the oil on the oil-immersion objective 3) Place the low-power objective in vertical position |
| Recall the difference between magnification and resolution | Magnification: enlarges the object being viewed Resolution: ability to distinguish detail |
| State why oil is used on a slide to be examined with the oil-immersion objective. | Prevents scattering of light rays passing through the object and to direct them straight upward through the lens. |
| Explain the advantage of parfocal lenses. | The object will remain in focus as you switch from one objective to another, preventing damage to the ocular lenses. |
| Why is focal length important when using the oil-immersion objective? | Prevents damage or scratching to the oil-immersion objective or damage to the slide. |
| Describe the best way to adjust the amount of light entering your specimen. | Adjusting the iris diaphragm of the condenser. |
| If a 5x ocular lens were used with your microscope, what maximum total magnification could be achieved? | 500x |
| When should the coarse and fine adjustment knobs be used? | With the low-power objective and the high-dry lens. |
| If you see moving particles in a hazy visual field, what steps can you take to obtain a clearer image? | Clean the objective and/or oculars with clean, dry lens paper. |
| What would you observe if you forgot to use oil with the oil-immersion lens? | Images that are not sharp or detailed. |
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