eEF1A1 coordinates different levels of HSP70 expression during heat stress to promote cell survival

1 eEF1A1 is a regulator of the adaptation to heat stress

1.1 Fig1a: eEF1A1 knockdown reduces Hsp70 expression during heat stress

1.2 Fig1b: eEF1A1 knockdown reduces cell survival during heat stress

2 eEF1A1 is a specific transcriptional regulator of HSP70 expression

2.1 Fig1c: eEF1A1 knockdown reduces Hsp70 mRNA expression during heat stress

2.2 Fig1d: eEF1A1 knockdown reduces RNA Pol II occupancy of an HSP70 gene (but not of an unrelated control gene)

2.3 Fig1e/f: eEF1A1 knockdown reduces the number and intensity of HSP70 transcription sites

3 eEF1A1 regulates HSF1 activity in transcriptionally active sites during heat stress, by interacting with it in a promoter-bound complex

3.1 eEF1A1 regulates HSF1 activity by interacting with it in a promoter-bound complex

3.1.1 eEF1A1 regulates HSF1 activity by interacting with it

3.1.1.1 eEF1A1 regulates HSF1 activity

3.1.1.1.1 Fig2a: eEF1A1 knockdown reduces binding of HSF1 to a synthetic HSP70 promoter fragment

3.1.1.1.2 Fig2b: eEF1A1 knockdown reduces HSF1 promoter occupancy in vivo

3.1.1.2 Fig2c: eEF1A1 interacts with HSF1 in vivo

3.1.1.3 Fig2d: eEF1A1-HSF1 complexes bind to a synthetic HSP70 promoter fragment

3.1.2 Fig2f: eEF1A1 itself binds to synthetic HSP70 promoter DNA

3.1.3 Fig2e: eEF1A1 specifically binds to HSP70 promoters in vitro

3.1.4 Fig3a-c: eEF1A1 localises to HSP70 transcription sites during heat stress in vivo

3.2 eEF1A1 is recruited to transcriptionally active sites during heat stress

3.2.1 Fig3d: eEF1A1 binding to HSP70 transcription sites is dependent on ongoing transcription

3.2.2 Fig3e: eEF1A1 directly binds to RNA pol II

4 eEF1A1 controls HSP70 translation by binding to it

4.1 Fig5d: eEF1A1 colocalises with HSP70 mRNA in the cytoplams during heat stress

4.2 Fig5e: eEF1A1 knockdown reduces HSP70 mRNA translation during heat stress

5 Fig5a-c: eEF1A1 knockdown impairs HSP70 mRNA export

6 eEF1A1 promotes HSP70 mRNA stability by binding to it

6.1 eEF1A1 binds to HSP70 mRNA

6.1.1 Fig4a,b: eEF1A1 remains bound to HSP70 mRNA following transcription

6.1.2 Fig4c: eEF1A1 binds to the 3'-UTR of HSP70 mRNA in vitro

6.2 eEF1A1 controls HSP70 mRNA stability

6.2.1 Fig4e: eEF1A1 knockdown reduces HSP70 mRNA half-life

6.2.2 Fig4f: eEF1A1 knckdown reduces the expression of a reporter gene containing the HSP70 promoter and 3'-UTR

Resource summary

	Created by Tobias von der Haar over 6 years ago