eEF1A1 coordinates different levels of HSP70 expression during heat stress to promote cell survival

Tobias von der Haar
Mind Map by Tobias von der Haar, updated more than 1 year ago
Tobias von der Haar
Created by Tobias von der Haar over 6 years ago
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Mind map from MSc student analysis of Vera et all

Resource summary

eEF1A1 coordinates different levels of HSP70 expression during heat stress to promote cell survival
1 eEF1A1 is a regulator of the adaptation to heat stress
1.1 Fig1a: eEF1A1 knockdown reduces Hsp70 expression during heat stress
1.2 Fig1b: eEF1A1 knockdown reduces cell survival during heat stress
2 eEF1A1 is a specific transcriptional regulator of HSP70 expression
2.1 Fig1c: eEF1A1 knockdown reduces Hsp70 mRNA expression during heat stress
2.2 Fig1d: eEF1A1 knockdown reduces RNA Pol II occupancy of an HSP70 gene (but not of an unrelated control gene)
2.3 Fig1e/f: eEF1A1 knockdown reduces the number and intensity of HSP70 transcription sites
3 eEF1A1 regulates HSF1 activity in transcriptionally active sites during heat stress, by interacting with it in a promoter-bound complex
3.1 eEF1A1 regulates HSF1 activity by interacting with it in a promoter-bound complex
3.1.1 eEF1A1 regulates HSF1 activity by interacting with it
3.1.1.1 eEF1A1 regulates HSF1 activity
3.1.1.1.1 Fig2a: eEF1A1 knockdown reduces binding of HSF1 to a synthetic HSP70 promoter fragment
3.1.1.1.2 Fig2b: eEF1A1 knockdown reduces HSF1 promoter occupancy in vivo
3.1.1.2 Fig2c: eEF1A1 interacts with HSF1 in vivo
3.1.1.3 Fig2d: eEF1A1-HSF1 complexes bind to a synthetic HSP70 promoter fragment
3.1.2 Fig2f: eEF1A1 itself binds to synthetic HSP70 promoter DNA
3.1.3 Fig2e: eEF1A1 specifically binds to HSP70 promoters in vitro
3.1.4 Fig3a-c: eEF1A1 localises to HSP70 transcription sites during heat stress in vivo
3.2 eEF1A1 is recruited to transcriptionally active sites during heat stress
3.2.1 Fig3d: eEF1A1 binding to HSP70 transcription sites is dependent on ongoing transcription
3.2.2 Fig3e: eEF1A1 directly binds to RNA pol II
4 eEF1A1 controls HSP70 translation by binding to it
4.1 Fig5d: eEF1A1 colocalises with HSP70 mRNA in the cytoplams during heat stress
4.2 Fig5e: eEF1A1 knockdown reduces HSP70 mRNA translation during heat stress
5 Fig5a-c: eEF1A1 knockdown impairs HSP70 mRNA export
6 eEF1A1 promotes HSP70 mRNA stability by binding to it
6.1 eEF1A1 binds to HSP70 mRNA
6.1.1 Fig4a,b: eEF1A1 remains bound to HSP70 mRNA following transcription
6.1.2 Fig4c: eEF1A1 binds to the 3'-UTR of HSP70 mRNA in vitro
6.2 eEF1A1 controls HSP70 mRNA stability
6.2.1 Fig4e: eEF1A1 knockdown reduces HSP70 mRNA half-life
6.2.2 Fig4f: eEF1A1 knckdown reduces the expression of a reporter gene containing the HSP70 promoter and 3'-UTR
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