Flashcards
Adhesion
Cards in this deck (7)
- 1What do sortases do?Surface display and cell wall anchoring of pili proteins
Cleave sorting signal (protein)
Joined to peptidoglycan precursors
Incorporated into cell wall
Protein (signal) stays attached to sortase - 2Describe the 3 adhesion steps (G +ve pilus)1. Pili interact w/ host cells' extracellular matrix
2. Zippering - bacterium pulled closer to cell surface
3. Pilus-mediated bacterial aggregation (more and more bacteria clump together forming microcolonies as interactions form b/w their cell surfaces - 3Describe the Gram negative pilus and its role, and give an exampleSmall tubular filaments (helical proteins)
Role: adhesion, aggregation, biofilm formation
e.g. Type IV pilus - 4Describe Neisseria gonorrhoeae's pilus activityNo zippering
T4 pilus (main anchorage) and opa bind to host receptors
Single interaction - pili retract; bacterium brought closer to host cell surface - 5What is opa?Opacity-associated proteins
- 6Describe twitching motility and give an example of an organism it is seen inNeisseria gonorrhoeae
1. Pilus extends (catalysed by assembly ATPase)
2. Pilus attaches to host epithelium
3. Pilus retracts (retraction ATPase involved)
4. Moves 1 micrometre/sec - 7Describe the T3SS. Give an e.g. of a species it's seen inEPEC
1. Channel forms b/w bacterial and host cell cytoplasm
2. Effectors (e.g. tir) inserted into host cell membrane
3. Intimin binds to tir; adhesion occurs
4. Other effectors interact w/ host cytoskeleton proteins and disassemble microvilli structure
5. Cytoskeleton reassembled into pedestal structure
Information
- Description
- Year 2 BIO258 - Microbes and Man Flashcards on Adhesion, created by Sophie Byrne on 06/10/2018.
- Created by
- Sophie Byrne
- Views
- 17
- Tags
- Rating