technique

technique

1. determining microbial taxonomy and phylogeny

   • classical characteristics
     • morphological characteristics
     • physiological and metabolic characteristics
     • biochemical characteristics
     • ecological characteristics
   • molecular characteristics
     • comparison of proteins sequences
     • nucleic acid base composition
       • how to determine GC content
         • Melting temperature Tm of DNA
         • HPLC
     • nucleic acid hybridization
     • Genomic fingerprinting
       • Multilocus sequence analysis(MLSA)
       • restriction fragment length plymorphism

2. Identification

   • growth and biochemical characteristics
   • bacteriophage typing
   • antibiotics susceptibility
   • microscopic examination
   • molecular methods
   • immunologic test