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Flashcards

BMBL

Jennifer Booth Veterinary Medicine Board Certification English 61 cards

Cards in this deck (61)

  1. 1Name 6 agents that only require approximately 10 organisms to cause infection.Bacillus anthracis
    Brucella spp.
    Francisella tularensis
    Mycobacterium tuberculosis complex
    Shiga toxin-producing E. coli
    Coxiella burnetii
  2. 2What is the family and genus of Hendra and Nipah viruses?Paramyxoviridae
    Henipavirus
  3. 3What is the natural host for Nipah and Hendra viruses? What intermediate host has caused human infections?Fruit bats in the genus Pteropus
    Pigs
  4. 4What BSL/ABSL level should work with Nipah or Hendra viruses be performed?4
  5. 5What type of disease does Hendra and Nipah viruses cause in humans?headache, fever, myalgia, encephalitis
    Respiratory disease in some
  6. 6How is macacine herpesvirus-1 transmitted to humans?Bites, scratches, or exposure of the mucous membranes to splashes when the animal is shedding from mucosal sites
    Asiatic macaques are the host
  7. 7At what BSL level can you manipulate tissues, cells, blood, or serum from macaques with appropriate personal protective equipment.2
  8. 8At which BSL level should you handle materials from which B virus is being cultured using appropriate personal protective equipment3
  9. 9Which BSL level should be used when propagating herpes B virus obtained from diagnostic samples or stocks4
  10. 10What type of BSL containment should B virus work with macaques be performed? What about work with small animal models?4 for all species
  11. 11Which poxvirus requires all work to be performed at BSL/ABSL-4 in a WHO facility only?Variola virus
  12. 12Which poxviruses should personnel be vaccinated for when working in or entering a lab area where activity with the viruses are being conducted?Vaccinia, monkeypox, cowpox
  13. 13What ABSL practices, containment equipment, and facilities are recommended for monkeypox work in experimentally or naturally infected animals.3
  14. 14BSL-__ facilities with BSL-__ practices are advised if vaccinated personnel perform non-animal work with monkeypox virus. (fill in the blanks)2, 3
  15. 15What BSL level does the NIH Guidelines recommend for manipulating attenuated vaccinia strains (modified virus Ankara [MVA], NYVAC, TROVAC, and ALVAC) in areas where no other human orthopoxviruses are being used.1
    Higher levels are recommended if other orthopoxviruses are present.
  16. 16What BSL level is recommended for work with Ebola?4 (also includes Ebola-Reston)
  17. 17What is the disease caused by hantavirus in America? In Europe & Asia?America: Hantavirus Pulmonary Syndrome
    Europe: Nephropathica endemica
    Asia: Hemorrhagic Fever and Renal Syndrome
  18. 18How is hantavirus transmitted to humans from rodents?Usually aerosol exposure to rodent urine
  19. 19What BSL practices, containment equipment, and facilities are recommended for laboratory handling of sera from persons potentially infected with hantaviruses.2
  20. 20Tissue samples potentially infected with hantavirus should be handled in BSL-__ facilities following BSL-__ practices and procedures. (fill in the blanks)2, 3
  21. 21Hantavirus cell-culture virus propagation and purification should be carried out in a BSL-__ facility using BSL-__ practices, containment equipment and procedures. (fill in the blanks)3, 3
  22. 22Experimental infections of rodent species known not to excrete hantavirus can be housed in ABSL-__ facilities using ABSL-__ practices and procedures. (fill in the blanks)2, 2
  23. 23Serum or tissue samples from rodents potentially infected with hantavirus should be handled at BSL-__ using BSL-__ practices, containment equipment and. (fill in the blanks)2, 3
  24. 24All work involving inoculation of hantavirus-containing samples into rodent species permissive for chronic infection should be conducted at ABSL-__.4
    (In North America, the deer mouse, the white-footed mouse, the
    rice rat, and the cotton rat are permissive for chronic infection.)
  25. 25T or F. Aerosols from, or direct skin contact with, cultures or with infectious clinical specimens from animals infected with hantavirus are commonly implicated in human infections.True
  26. 26What BSL practices, containment equipment, and facilities are recommended for routine clinical specimens of human or animal origin infected with Brucella2
  27. 27Products of conception containing or believed to contain pathogenic Brucella should be handled at which BSL level? Why?3
    Due to high concentration of organism per gram of tissue
  28. 28BSL-__ and ABSL-__ practices, containment equipment, and facilities are recommended, for all manipulations of cultures of pathogenic Brucella spp. and for experimental animal studies.3, 3
  29. 29BSL-__ practices, containment equipment, and facilities are recommended for activities involving the necropsy of infected birds and the diagnostic examination of tissues or cultures known to contain or be potentially infected with C. psittaci strains of avian origin. (fill in the blank)3
  30. 30Activities involving non-avian strains of C. psittaci may be performed in a BSL-__ facility with BSL-__ practices. (fill in the blanks)2, 3
  31. 31ABSL-__ practices, containment equipment, and facilities and respiratory protection are recommended for personnel working with caged birds naturally or experimentally infected with Chlamydia psittaci.3
  32. 32What BSL/ABSL level is recommended for work with Clostridium botulinum?2
  33. 33BSL-__ practices, containment equipment, and facilities are recommended for activities involving clinical materials of human or animal origin suspected or known to contain F. tularensis. (fill in the blank)2
  34. 34BSL-__ and ABSL-__ practices, containment equipment, and facilities are recommended for all manipulations of suspect cultures, animal necropsies and for experimental animal studies known to be infected with F. tularensis.3, 3
  35. 35For subspecies in the Mycobacterium tubercoulosis complex, BSL-__ practices and procedures, containment equipment, and facilities are required for non-aerosol-producing manipulations of clinical specimens such as preparation of acid-fast smears.2
  36. 36BSL-__ practices, containment equipment, and facilities are required for laboratory activities in the propagation and manipulation of cultures of any of the subspecies of the M. tuberculosis complex.3
  37. 37BSL-__ practices, containment equipment, and facilities are required for animal studies using experimentally or naturally infected NHP with any of the subspecies of the M. tuberculosis complex3
  38. 38Animal studies using guinea pigs or mice experimentally infected with any subspecies of the M. tuberculosis complex can be conducted at ABSL-__.2
  39. 39BSL-__ practices, containment equipment, and facilities are recommended for all activities involving the handling of potentially infectious clinical materials and cultures of Yersinia pestis.2
  40. 40All work, including necropsies of animals potentially infected with Y. pestis should be performed in at BSL-__.2
  41. 41For Y. pestis, BSL-__ is recommended for activities with high potential for droplet or aerosol production, and for activities involving large-scale production or high concentrations of infectious materials.3
  42. 42How do you determine if experimental animal infections with Y. pestis should be conducted at ABSL-2 or -3?Perform a risk assessment
  43. 43BSL-__ facilities and arthropod containment level __ practices are recommended for all laboratory work involving arthropods infected with Y. pestis.3, 3
  44. 44When is BSL-3 practices, containment equipment, and facilities required for work with Blastomyces dermatiditis?When handling sporulating mold-form cultures and soil or other environmental samples known
    or likely to contain infectious conidia.
  45. 45What ABSL should animal work with Blastomyces dermatiditis be conducted?ABSL-2
    (infections are not communicable)
  46. 46When should work with Coccidioides immitis be performed under BSL-3 facilities, practices, and containment?When propagating and manipulating sporulating cultures already identified as
    Coccidioides spp. and for processing soil or other environmental materials
    known to contain infectious arthroconidia. (vegetative, BSL-2 is OK)
  47. 47What determines if ABSL-2 or -3 should be used for animals experimentally infected with C. immitis?The route of infection:
    Parenteral = ABSL2
    Intranasal or pulmonary = ABSL3
  48. 48BSL-__ practices and facilities are recommended for nonpropagative laboratory procedures with Coxiella burnetii, including serological examinations and staining of impression smears2
  49. 49BSL-__ practices and facilities are recommended for activities involving the inoculation, incubation, and harvesting of embryonated eggs or cell cultures, the necropsy of infected animals and the manipulation of infected tissues with C. burnetii.3
  50. 50What ABSL should experimental infections of animals with C. burnetii be performed?3
    (including rodents, excrete in urine and feces)
  51. 51What determines if work with samples infected with Rickettsia spp. should be performed at BSL-2 or -3?All non-propogative lab work can be BSL2.
    Any work with tissues or propagation must be BSL3.
  52. 52What determines if work with animals experimentally infected with Rickettsia spp. should be conducted at ABSL-2 or -3?All non-arthropod animal work can be ABSL2.
    All infected arthropods must be ABSL3
  53. 53When working with LCMV, BSL-__ practices, containment equipment, and facilities are suitable for activities utilizing known or potentially infectious body fluids, and for cell culture passage of laboratory-adapted strains.2
  54. 54BSL-__ is required when manipulating infected transplantable tumors, field isolates and clinical materials from human cases of LCMV infection.3
  55. 55Strains of LCMV that are shown to be lethal in non-human primates should be handled at BSL-__.3
  56. 56ABSL-__ practices, containment equipment, and facilities are suitable for LCMV studies in adult mice with mouse brain-passaged strains requiring BSL-2 containment.2
  57. 57What ABSL level should LCMV work with hamster be performed at?3
  58. 58What determines if non-animal work with HIV or SIV should be conducted at BSL-2 or -3?Production of lab-scale quantities may be at BSL2.
    Large-scale quantities of concentrated HIV or SIV must be BSL3.
  59. 59What ABSL level must work with HIV or SIV in NHP or other animals be conducted?2
  60. 60What BSL level must NHP Quarantine be?2
  61. 61Animal work with VEE virus, EEE virus and WEE virus should be performed under ABSL-__ conditions.3

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Board Certification Veterinary Medicine (Laboratory Animal Medicine) Flashcards on BMBL, created by Jennifer Booth on 14/02/2018.
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Jennifer Booth
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