Flashcards
BMS02-1010 Enzyme Kinetics
Cards in this deck (17)
- 1What is KCat?The turnover number: number of substrate molecules converted to products in a certain amount of time
- 2What is the substrate vs reaction graph? How does it change as the concentration increasesConcentration of substrate on the x axis against rxn
At low concentrations, substrate proportional to rxn rate: 1st order
At high concentrations, rxn rate independent of substrate: 0 order - 3What is the Michaelis Menton reaction? What do K1, K-1, and K2 stand for?E+S<-->ES-->E+P
K1: E+S-->ES
K-1: E+S<--ES
K2: ES--> E+P - 4What are 5 assumptions of the Michaelis Menton reaction?1. [substrate]>>>[enzyme]
2. [ES] doesn't change
3. Initial velocity used
4. P is small enough to be negligible
5. ES<--E+P ignored - 5What is the Michaelis Menton equation?V= Vmax[S]/Km+[S]
- 6What does Km represent in terms of Vmax? What about affinity?It is the concentration of substrate when Vmax 1/2.
A lower Km indicates a higher affinity - 7K2....K-1 so rxn to E+P neglibaleK2<<<
- 8What is Kd?The dissociation constant of ES aka K2
- 9What do phosphorylases do?Transfer phosphate group from inorganic molecule to molecule
- 10How does protein phosphorylation affect glycogen phosphorylase and glycogen synthase?Phosphorylation activates glycogen phosphorylase and inactivates glycogen synthase
- 11What does phosphatase do?Uses H20 to cleave Phosphate from molecule
- 12On a graph, what does a competitive inhibitor change and not change? What about noncompetitive inhibitor?-Increases Km but not Vmax bc can be overcome
-Decreases Vmax but not Km - 13What is the difference between allosteric and noncompetitive enzymes?Noncompetitive poisons/damages enzyme
- 14What is an example of negative allosteric regulation on phosphofructokinase?ATP/Citrate on Phosphofructokinase
As a result, Fructose 6 phosphate does not become Fructose 1,6 biphosphate - 15What is an example of positive allosteric regulation on pyruvate kinase?Fructose 1,6-biphosphate and phosphoenol pyruvate increase its activity
More conversion of phosphoenol pyruvate-->pyruvate as a result - 16On the Lineweaver Burke plot, the Y intercept represents.... The X intercept represents....Y intercept: 1/Vmax (changes w allosteric)
X intercept: -1/Km (changes w competitive) - 17What is an example of a isoenzyme?Lactate dehydrogenase- has 4 monomers
Each one can be heart/muscle type
Information
- Description
- BMS02 - Molecules and Cells Flashcards on BMS02-1010 Enzyme Kinetics, created by Evian Chai on 02/05/2020.
- Created by
- Evian Chai · May 2020
- Updated
- May 2, 2020
- Views
- 12
- Tags
- Rating