Flashcards
Protein Secretion & The SecY Pathway
Cards in this deck (25)
- 1SecYUbiquitous protein that uses SecYeg channels in the membrane to secrete proteins
- 2TAT (Twin Argernine System)Transport of fully folded proteins (inner membrane only)
- 3Secretion Systems (Types I-IV & VI)Inner & Outer Membrane transport (can be Sec independent or Sec requiring)
- 4Type V AutotransporterLike Types 1-IV & VI, often encoded by pathogenicity islands
- 5SecY- Protein Sorting/TargetingAn N-terminal targeting signal sequence
- 6SecY- SecBFor exported proteins
- 7SecY- SRPSignal recognition particle (for membrane proteins)
- 8SecY- Translocation Protein ChannelSecY, E and Gamma
- 9SecY-SecAATPase that provides energy for translocation
- 10SecY- ReleaseSignal sequence is cleaved by peptidases and proteins fold
- 11SecY-ChannelOccurs as a partially active dimer (one monomer active, one inactive)
- 121) Sec B dimer binds the protein and...brings it to SecY channel
- 132) The 'plug' keeps SecY closed until...it interacts with the signal sequence
- 143) ATP hydrolysis causes....SecA to pump the protein through the SecY channel
- 15SecB Dependent- DestinationOutside of the cell or the outer cell membrane
- 16SecB Dependent- Is the protein foldedNo
- 17SecB Dependent- Channel TargetSec A
- 18SRP Dependent- DestinationMembrane itself
- 19SRP Dependent- Ribosome bindingYes
- 20SRP Dependent- Channel TargetFTSY
- 21SRP Dependent- FoldedNo- is translated directly through channel
- 22Sec Y Signal Peptide- Length25 amino acids
- 23Sec Y Signal Peptide- N Region+ve
- 24Sec Y Signal Peptide- H RegionHydrophobic
- 25Sec Y Signal Peptide- C RegionAXA sequence immediately preceeding cleavage site
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- Medical Microbiology (Protein Secretion) Flashcards on Protein Secretion & The SecY Pathway, created by gina_evans0312 on 01/03/2014.
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